Product Name
TBX22, siRNA
Full Product Name
TBX22 siRNA (Mouse)
Product Synonym Names
T-box transcription factor TBX22; T-box protein 22
Product Gene Name
TBX22 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8K402
Specificity
TBX22 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TBX22 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TBX22 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TBX22 sirna
siRNA to inhibit TBX22 expression using RNA interference
Applications Tested/Suitable for TBX22 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TBX22. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001277676.1
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NCBI GenBank Nucleotide #
NM_001290747.1
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UniProt Primary Accession #
Q8K402
[Other Products]
UniProt Related Accession #
Q8K402[Other Products]
Molecular Weight
58,498 Da
NCBI Official Full Name
T-box transcription factor TBX22 isoform 3
NCBI Official Synonym Full Names
T-box 22
NCBI Official Symbol
Tbx22??[Similar Products]
NCBI Official Synonym Symbols
D230020M15Rik
??[Similar Products]
NCBI Protein Information
T-box transcription factor TBX22
UniProt Protein Name
T-box transcription factor TBX22
Protein Family
T-box transcription factor
UniProt Gene Name
Tbx22??[Similar Products]
UniProt Synonym Gene Names
T-box protein 22??[Similar Products]
UniProt Entry Name
TBX22_MOUSE
UniProt Comments for TBX22
TBX22: Probable transcriptional regulator involved in developmental processes. This is major determinant crucial to palatogenesis. Defects in TBX22 are the cause of X-linked cleft palate with ankyloglossia (CPX). 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Cellular Component: nucleus
Molecular Function: DNA binding; transcription factor activity
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
Research Articles on TBX22
1. Results demonstrate that FGF8 induces Tbx22 in the early face while BMP4 represses and thus restricts its expression. This regulation is conserved between chicken and mouse.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
