Product Name
RAB3GAP2, siRNA
Full Product Name
RAB3GAP2 siRNA (Rat)
Product Synonym Names
Rab3 GTPase-activating protein non-catalytic subunit; Rab3 GTPase-activating protein 150 kDa subunit; Rab3-GAP p150; Rab3-GAP150; Rab3-GAP regulatory subunit
Product Gene Name
RAB3GAP2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q5U1Z0
Specificity
RAB3GAP2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat RAB3GAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RAB3GAP2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RAB3GAP2 sirna
siRNA to inhibit RAB3GAP2 expression using RNA interference
Applications Tested/Suitable for RAB3GAP2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RAB3GAP2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001035244.2
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NCBI GenBank Nucleotide #
NM_001040154.2
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UniProt Primary Accession #
Q5U1Z0
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UniProt Related Accession #
Q5U1Z0[Other Products]
Molecular Weight
154,431 Da
NCBI Official Full Name
rab3 GTPase-activating protein non-catalytic subunit
NCBI Official Synonym Full Names
RAB3 GTPase activating protein subunit 2
NCBI Official Symbol
Rab3gap2??[Similar Products]
NCBI Official Synonym Symbols
RGD1311518
??[Similar Products]
NCBI Protein Information
rab3 GTPase-activating protein non-catalytic subunit
UniProt Protein Name
Rab3 GTPase-activating protein non-catalytic subunit
UniProt Synonym Protein Names
Rab3 GTPase-activating protein 150 kDa subunit; Rab3-GAP p150; Rab3-GAP150; Rab3-GAP regulatory subunit
Protein Family
Rab3 GTPase-activating protein
UniProt Gene Name
Rab3gap2??[Similar Products]
UniProt Synonym Gene Names
Rab3-GAP150??[Similar Products]
UniProt Entry Name
RBGPR_RAT
UniProt Comments for RAB3GAP2
RAB3GAP2: Regulatory subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab3 GTPase-activating complex specifically converts active Rab3-GTP to the inactive form Rab3- GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non- synaptic vesicular release of neurotransmitters. Defects in RAB3GAP2 are the cause of Martsolf syndrome (MARTS). Martsolf syndrome is characterized by congenital cataracts, mental retardation, and hypogonadism. Inheritance is autosomal recessive. Defects in RAB3GAP2 are the cause of Warburg micro syndrome type 2 (WARBM2). WARBM2 is a rare syndrome characterized by microcephaly, microphthalmia, microcornia, congenital cataracts, optic atrophy, cortical dysplasia, in particular corpus callosum hypoplasia, severe mental retardation, spastic diplegia, and hypogonadism. Belongs to the Rab3-GAP regulatory subunit family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: GAPs, Rab; GAPs
Cellular Component: protein complex; cytoplasm; plasma membrane
Molecular Function: protein heterodimerization activity; Rab guanyl-nucleotide exchange factor activity; enzyme regulator activity; Rab GTPase binding; GTPase activator activity
Biological Process: regulation of GTPase activity; positive regulation of GTPase activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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