Product Name
HMGCL, siRNA
Full Product Name
HMGCL siRNA (Mouse)
Product Synonym Names
Hydroxymethylglutaryl-CoA lyase mitochondrial; HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase
Product Gene Name
HMGCL sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P38060
Specificity
HMGCL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HMGCL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HMGCL sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HMGCL sirna
siRNA to inhibit HMGCL expression using RNA interference
Applications Tested/Suitable for HMGCL sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HMGCL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032280.2
[Other Products]
NCBI GenBank Nucleotide #
NM_008254.2
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UniProt Primary Accession #
P38060
[Other Products]
UniProt Secondary Accession #
Q8QZS6[Other Products]
UniProt Related Accession #
P38060[Other Products]
Molecular Weight
34,239 Da
NCBI Official Full Name
hydroxymethylglutaryl-CoA lyase, mitochondrial
NCBI Official Synonym Full Names
3-hydroxy-3-methylglutaryl-Coenzyme A lyase
NCBI Official Symbol
Hmgcl??[Similar Products]
NCBI Official Synonym Symbols
HL; AW476067
??[Similar Products]
NCBI Protein Information
hydroxymethylglutaryl-CoA lyase, mitochondrial
UniProt Protein Name
Hydroxymethylglutaryl-CoA lyase, mitochondrial
UniProt Synonym Protein Names
3-hydroxy-3-methylglutarate-CoA lyase
UniProt Gene Name
Hmgcl??[Similar Products]
UniProt Synonym Gene Names
HL; HMG-CoA lyase??[Similar Products]
UniProt Entry Name
HMGCL_MOUSE
UniProt Comments for HMGCL
HMGCL: Key enzyme in ketogenesis (ketone body formation). Terminal step in leucine catabolism. Defects in HMGCL are the cause of 3-hydroxy-3- methylglutaryl-CoA lyase deficiency (HMGCLD); also known as hydroxymethylglutaricaciduria or HL deficiency. An autosomal recessive disease affecting ketogenesis and L-leucine catabolism. The disease usually appears in the first year of life after a fasting period and its clinical acute symptoms include vomiting, seizures, metabolic acidosis, hypoketotic hypoglycemia and lethargy. These symptoms sometimes progress to coma, with fatal outcome in some cases. Belongs to the HMG-CoA lyase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - synthesis and degradation of ketone bodies; Mitochondrial; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Lyase; EC 4.1.3.4
Cellular Component: mitochondrion; mitochondrial inner membrane; peroxisome
Molecular Function: protein homodimerization activity; hydroxymethylglutaryl-CoA lyase activity; acyl-CoA binding; carboxylic acid binding; metal ion binding; manganese ion binding; lyase activity; magnesium ion binding; catalytic activity; receptor binding
Biological Process: mitochondrion organization and biogenesis; embryonic development; acyl-CoA metabolic process; ketone body biosynthetic process; protein tetramerization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
