For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of P2Y12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS746396 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Purinergic receptor P2Y, G-protein coupled, 12 (P2Y12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing P2Y12. The ELISA analytical biochemical technique of the MBS746396 kit is based on P2Y12 antibody-P2Y12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect P2Y12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, P2Y12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: P2Y12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-P2Y12 antibody and an P2Y12-HRP conjugate. The assay sample and buffer are incubated together with P2Y12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P2Y12 concentration since P2Y12 from samples and P2Y12-HRP conjugate compete for the anti-P2Y12 antibody . Since the number of sites is limited, as more sites are occupied by P2Y12 from the sample, fewer sites are left to bind P2Y12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P2Y12 concentration in each sample is interpolated from this standard curve.

39,439 Da

purinergic receptor P2Y, G-protein coupled, 12

P2Y purinoceptor 12; ADP-glucose receptor; purinergic receptor P2RY12; P2Y12 platelet ADP receptor; Gi-coupled ADP receptor HORK3; G-protein coupled receptor SP1999; putative G-protein coupled receptor

P2Y purinoceptor 12

HORK3; P2Y12; ADPG-R; P2Y(ADP)??[Similar Products]

P2Y12_HUMAN

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelet aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Mutations in this gene are implicated in bleeding disorder, platelet type 8 (BDPLT8). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

P2Y12: Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Involved in platelets aggregation. Defects in P2RY12 are the cause of bleeding disorder platelet-type 8 (BDPLT8). A condition characterized by mild to moderate mucocutaneous bleeding, and excessive bleeding after surgery or trauma. The defect is due to severe impairment of platelet response to ADP resulting in defective platelet aggregation. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Mitochondrial; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: cell surface; mitochondrion; integral to plasma membrane; plasma membrane; basal plasma membrane; intrinsic to membrane; caveola; external side of plasma membrane

Molecular Function: guanyl-nucleotide exchange factor activity; adenosine receptor activity, G-protein coupled; platelet ADP receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; platelet activation; negative regulation of cell differentiation; cell projection organization and biogenesis; hemostasis; protein kinase B signaling cascade; positive regulation of ion transport; G-protein signaling, adenylate cyclase inhibiting pathway; regulation of calcium ion transport; blood coagulation; adenosine receptor signaling pathway; positive regulation of GTPase activity

Disease: Bleeding Disorder, Platelet-type, 8

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