Anti -Neurofilament M 145-160kD (NF-M)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 14; NC_000080.5 (68737602..68743061, complement). Location: 14 D1; 14

Monoclonal

IgG1

6H9

Mouse

The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species. Recognizes intact NF-M strongly and specifically on Western Blots in all mammals and birds tested to date and also binds NF-M in cells in tissue culture and in sectioned material.

Supernatant
Tissue culture supernatant

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%)aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-Nefm antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160kD, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Antibodies; Abs to Neurofilament

Western Blot (WB), Immunofluorescence (IF)

Suitable for use in Immunofluorescence, Western Blotting.
Dilution: Immunofluorescence: 1:100
Western Blotting: 1:1000

95,916 Da[Similar Products]

neurofilament, medium polypeptide

neurofilament medium polypeptide; neurofilament-M; OTTMUSP00000017611; neurofilament 3, medium; neurofilament protein M; 160 kDa neurofilament protein; neurofilament triplet M protein

Neurofilament medium polypeptide

Nef3; Nfm??[Similar Products]

NFM_MOUSE

NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function.

Protein type: Cytoskeletal

Cellular Component: neuron projection; cytoskeleton; axon; cell; neurofilament; intermediate filament; intracellular; neuromuscular junction; myelin sheath

Molecular Function: structural molecule activity

Biological Process: neurofilament cytoskeleton organization and biogenesis; regulation of axon diameter; axon cargo transport; intermediate filament cytoskeleton organization and biogenesis; intermediate filament bundle assembly; microtubule cytoskeleton organization and biogenesis

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.