Anti -Neurofilament M 160-200kD (NF-M)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 14; NC_000080.5 (68737602..68743061, complement). Location: 14 D1; 14

Monoclonal

IgG1

3G201

Mouse

Recognizes rat NF-M. Species Crossreactivity: Human, mouse, chicken and rabbit.

Affinity Purified
Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-Nefm antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Neurofilaments (NFs) are prominent components of large myelinated axons. Based on sequence homology and intron placement, the NF genes have been classified as type IV IFs along with alpha-internexin, which is also expressed in the nervous system. NFs may function as effector proteins subject to a complex regulatory cross-talk between neurons and myelinating Schwann cells or oligrodendrocytes. Mammalian neurofilaments are assembled from light (NF-L), mid-sized (NF-M), and heavy (NF-H) neurofilament proteins. NF-M is required if myelinated axons are to achieve maximal diameters and NF-M has been shown to regulate the level of NF-L. NF-M subunits mainly regulate axonal caliber by altering the level of NF-L and in turn the number of NFs in the axon. Results seem to explain as a perturbation of NF stoichiometry in which overexpression of NF-M leads to the formation of perikaryal NF inclusions in motor neurons and the depletion of NFs from axons. Overexpression of NF-M in combination with NF-L increased radial growth of axons, which suggests that NF-M or NF-H must coassemble into filaments with NF-L before they can affect the radial growth of axons. The immature form of NFs composed of NF-L and NF-M, might function in establishing the early neuronal phenotype and in maintaining neurite outgrowth.

Antibodies; Abs to Neurofilament

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 5-10ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1-5ug/ml
Immunohistochemistry (FFPE & Frozen Sections): 10ug/ml

95,916 Da[Similar Products]

neurofilament, medium polypeptide

neurofilament medium polypeptide; neurofilament-M; OTTMUSP00000017611; neurofilament 3, medium; neurofilament protein M; 160 kDa neurofilament protein; neurofilament triplet M protein

Neurofilament medium polypeptide

Nef3; Nfm??[Similar Products]

NFM_MOUSE

NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function.

Protein type: Cytoskeletal

Cellular Component: neuron projection; cytoskeleton; cell; axon; neurofilament; intermediate filament; intracellular; neuromuscular junction; myelin sheath

Molecular Function: structural molecule activity

Biological Process: neurofilament cytoskeleton organization and biogenesis; axon cargo transport; regulation of axon diameter; intermediate filament cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; intermediate filament bundle assembly

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