Mouse Glutaredoxin-related protein 5; mitochondrial (GLRX5) ELISA kit; C14orf87; FLB4739; GRX5; MGC14129; PR01238; PRO1238; glutaredoxin 5 homolog; glutaredoxin 5

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of mouse GLRX5. No significant cross-reactivity or interference between mouse GLRX5 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of GLRX5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS917136 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutaredoxin 5 (GLRX5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GLRX5. The ELISA analytical biochemical technique of the MBS917136 kit is based on GLRX5 antibody-GLRX5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GLRX5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GLRX5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GLRX5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLRX5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLRX5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLRX5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

16,292 Da

glutaredoxin 5 homolog (S. cerevisiae)

glutaredoxin-related protein 5, mitochondrial; monothiol glutaredoxin-5

Glutaredoxin-related protein 5, mitochondrial

GLRX5_MOUSE

GLRX5: Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Required for normal iron homeostasis. Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1. Defects in GLRX5 are a cause of anemia sideroblastic pyridoxine-refractory autosomal recessive (PRARSA). A form of sideroblastic anemia not responsive to pyridoxine. Sideroblastic anemia is characterized by anemia of varying severity, hypochromic peripheral erythrocytes, systemic iron overload secondary to chronic ineffective erythropoiesis, and the presence of bone marrow ringed sideroblasts. Sideroblasts are characterized by iron-loaded mitochondria clustered around the nucleus. Belongs to the glutaredoxin family. Monothiol subfamily.

Protein type: Oxidoreductase

Cellular Component: mitochondrion; cell soma; dendrite; nucleus

Molecular Function: 2 iron, 2 sulfur cluster binding; electron carrier activity; metal ion binding; iron-sulfur cluster binding; protein disulfide oxidoreductase activity

Biological Process: cell redox homeostasis

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