Product Name
CDK5RAP2, siRNA
Full Product Name
CDK5RAP2 siRNA (Mouse)
Product Synonym Names
KIAA1633; CDK5 regulatory subunit-associated protein 2; CDK5 activator-binding protein C48
Product Gene Name
CDK5RAP2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8K389
Specificity
CDK5RAP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CDK5RAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CDK5RAP2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CDK5RAP2 sirna
siRNA to inhibit CDK5RAP2 expression using RNA interference
Applications Tested/Suitable for CDK5RAP2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CDK5RAP2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_666102.2
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NCBI GenBank Nucleotide #
NM_145990.3
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UniProt Primary Accession #
Q8K389
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UniProt Secondary Accession #
Q6PCN1; Q6ZPL0; A2AVA1[Other Products]
UniProt Related Accession #
Q8K389[Other Products]
Molecular Weight
205,945 Da
NCBI Official Full Name
CDK5 regulatory subunit-associated protein 2
NCBI Official Synonym Full Names
CDK5 regulatory subunit associated protein 2
NCBI Official Symbol
Cdk5rap2??[Similar Products]
NCBI Official Synonym Symbols
an; mKIAA1633; 2900018K03Rik
??[Similar Products]
NCBI Protein Information
CDK5 regulatory subunit-associated protein 2
UniProt Protein Name
CDK5 regulatory subunit-associated protein 2
UniProt Synonym Protein Names
CDK5 activator-binding protein C48
Protein Family
CDK5 regulatory subunit-associated protein
UniProt Gene Name
Cdk5rap2??[Similar Products]
UniProt Synonym Gene Names
Kiaa1633??[Similar Products]
UniProt Entry Name
CK5P2_MOUSE
UniProt Comments for CDK5RAP2
CDK5RAP2: Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation. Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3). A disorder defined as a head circumference more than 3 standard deviations below the age- related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal
Cellular Component: spindle pole; Golgi apparatus; centrosome; microtubule; pericentriolar material; cytoskeleton; cell; perinuclear region of cytoplasm; cytoplasm; cell junction
Molecular Function: calmodulin binding; tubulin binding; protein binding; microtubule binding; protein complex binding; protein kinase binding
Biological Process: negative regulation of neuron differentiation; establishment of mitotic spindle orientation; neurogenesis; centrosome organization and biogenesis; positive regulation of transcription, DNA-dependent; microtubule cytoskeleton organization and biogenesis; brain development; negative regulation of centriole replication; microtubule bundle formation; chromosome segregation
Research Articles on CDK5RAP2
1. Mouse expression pattern of CDK5RAP2 is similar to that seen in humans and is in concordance with pathology suggested by neuroimaging studies in humans and mouse
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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