Product Name
GRIK3, siRNA
Full Product Name
GRIK3 siRNA (Human)
Product Synonym Names
GLUR7; Glutamate receptor ionotropic. kainate 3; GluK3; Excitatory amino acid receptor 5; EAA5; Glutamate receptor 7; GluR-7; GluR7
Product Gene Name
GRIK3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13003
Specificity
GRIK3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GRIK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GRIK3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GRIK3 sirna
siRNA to inhibit GRIK3 expression using RNA interference
Applications Tested/Suitable for GRIK3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GRIK3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000822.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000831.3
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UniProt Primary Accession #
Q13003
[Other Products]
UniProt Secondary Accession #
Q13004; Q16136; A9Z1Z8; B1AMS6[Other Products]
UniProt Related Accession #
Q13003[Other Products]
Molecular Weight
98,727 Da
NCBI Official Full Name
glutamate receptor ionotropic, kainate 3
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, kainate 3
NCBI Official Symbol
GRIK3??[Similar Products]
NCBI Official Synonym Symbols
EAA5; GLR7; GLUR7; GluK3; GluR7a
??[Similar Products]
NCBI Protein Information
glutamate receptor ionotropic, kainate 3
UniProt Protein Name
Glutamate receptor ionotropic, kainate 3
UniProt Synonym Protein Names
Excitatory amino acid receptor 5; EAA5; Glutamate receptor 7; GluR-7; GluR7
Protein Family
Glutamate receptor ionotropic
UniProt Gene Name
GRIK3??[Similar Products]
UniProt Synonym Gene Names
GLUR7; GluK3; EAA5; GluR-7; GluR7??[Similar Products]
UniProt Entry Name
GRIK3_HUMAN
NCBI Summary for GRIK3
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. It is not certain if the subunit encoded by this gene is subject to RNA editing as the other 2 family members (GRIK1 and GRIK2). A Ser310Ala polymorphism has been associated with schizophrenia, and there are conflicting reports of its association with the pathogenesis of delirium tremens in alcoholics. [provided by RefSeq, Jul 2008]
UniProt Comments for GRIK3
GluR7: Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate >> L-glutamate = quisqualate >> AMPA = NMDA. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK3 subfamily.
Protein type: Membrane protein, integral; Channel, ligand-gated; Receptor, misc.; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1p34.3
Cellular Component: dendrite cytoplasm; presynaptic membrane; postsynaptic membrane; kainate selective glutamate receptor complex; integral to plasma membrane; axon; dendrite; plasma membrane; terminal button; perikaryon; cell junction
Molecular Function: adenylate cyclase inhibiting metabotropic glutamate receptor activity; extracellular-glutamate-gated ion channel activity; kainate selective glutamate receptor activity; ionotropic glutamate receptor activity; glutamate receptor activity
Biological Process: synaptic transmission, glutamatergic; synaptic transmission; regulation of membrane potential; ionotropic glutamate receptor signaling pathway; glutamate signaling pathway; metabotropic glutamate receptor signaling pathway; negative regulation of synaptic transmission, glutamatergic; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway
Research Articles on GRIK3
1. 1p34.3 deletion involving GRIK3: Further clinical implication of GRIK family glutamate receptors in the pathogenesis of developmental delay.
Precautions
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Disclaimer
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