Product Name
GRIK3, siRNA
Full Product Name
GRIK3 siRNA (Mouse)
Product Synonym Names
GLUR7; Glutamate receptor ionotropic. kainate 3; GluK3; Glutamate receptor 7; GluR-7; GluR7
Product Gene Name
GRIK3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for B1AS29
Specificity
GRIK3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GRIK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GRIK3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GRIK3 sirna
siRNA to inhibit GRIK3 expression using RNA interference
Applications Tested/Suitable for GRIK3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GRIK3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001074566.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001081097.2
[Other Products]
UniProt Primary Accession #
B1AS29
[Other Products]
UniProt Secondary Accession #
Q17R20; Q9JIA8[Other Products]
UniProt Related Accession #
B1AS29[Other Products]
Molecular Weight
104,051 Da
NCBI Official Full Name
glutamate receptor ionotropic, kainate 3
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, kainate 3
NCBI Official Symbol
Grik3??[Similar Products]
NCBI Official Synonym Symbols
GluK3; Glur7; Glur-7; GluR7-3; 9630027E11
??[Similar Products]
NCBI Protein Information
glutamate receptor ionotropic, kainate 3
UniProt Protein Name
Glutamate receptor ionotropic, kainate 3
UniProt Synonym Protein Names
Glutamate receptor 7; GluR-7; GluR7
Protein Family
Glutamate receptor ionotropic
UniProt Gene Name
Grik3??[Similar Products]
UniProt Synonym Gene Names
Glur7; GluK3; GluR-7; GluR7??[Similar Products]
UniProt Entry Name
GRIK3_MOUSE
NCBI Summary for GRIK3
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Transcript variants encoding different isoforms have been described for this gene, however, their full-length nature is not known. [provided by RefSeq, Jul 2008]
UniProt Comments for GRIK3
GluR7: Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate >> L-glutamate = quisqualate >> AMPA = NMDA. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK3 subfamily.
Protein type: Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass; Receptor, misc.
Cellular Component: dendrite cytoplasm; kainate selective glutamate receptor complex; integral to plasma membrane; dendrite; integral to membrane; terminal button; perikaryon; presynaptic membrane; postsynaptic membrane; membrane; axon; plasma membrane; synapse; cell junction
Molecular Function: adenylate cyclase inhibiting metabotropic glutamate receptor activity; protein binding; extracellular-glutamate-gated ion channel activity; kainate selective glutamate receptor activity; ionotropic glutamate receptor activity; ion channel activity; receptor activity; glutamate receptor activity
Biological Process: synaptic transmission, glutamatergic; regulation of membrane potential; transport; ionotropic glutamate receptor signaling pathway; glutamate signaling pathway; metabotropic glutamate receptor signaling pathway; negative regulation of synaptic transmission, glutamatergic; ion transport; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway; signal transduction
Research Articles on GRIK3
1. Polarized trafficking of spliced variant GluK3b in neurons may be controlled by the regulation of receptor endocytosis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
