Full Product Name
TPP1 siRNA (Human)
Product Synonym Names
CLN2; Tripeptidyl-peptidase 1; TPP-1; Cell growth-inhibiting gene 1 protein; Lysosomal pepstatin-insensitive protease; LPIC; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase I; TPP-I
Product Gene Name
TPP1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O14773
Specificity
TPP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TPP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TPP1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TPP1 sirna
siRNA to inhibit TPP1 expression using RNA interference
Applications Tested/Suitable for TPP1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TPP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000382.3
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NCBI GenBank Nucleotide #
NM_000391.3
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UniProt Primary Accession #
O14773
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UniProt Secondary Accession #
Q53HT1; Q5JAK6; Q6UX56; Q71JP6; Q96C37[Other Products]
UniProt Related Accession #
O14773[Other Products]
Molecular Weight
34,464 Da
NCBI Official Full Name
tripeptidyl-peptidase 1 preproprotein
NCBI Official Synonym Full Names
tripeptidyl peptidase I
NCBI Official Symbol
TPP1??[Similar Products]
NCBI Official Synonym Symbols
CLN2; GIG1; LPIC; SCAR7; TPP-1
??[Similar Products]
NCBI Protein Information
tripeptidyl-peptidase 1
UniProt Protein Name
Tripeptidyl-peptidase 1
UniProt Synonym Protein Names
Cell growth-inhibiting gene 1 protein; Lysosomal pepstatin-insensitive protease; LPIC; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase I; TPP-I
Protein Family
Tripeptidyl-peptidase
UniProt Gene Name
TPP1??[Similar Products]
UniProt Synonym Gene Names
CLN2; TPP-1; LPIC; TPP-I??[Similar Products]
UniProt Entry Name
TPP1_HUMAN
NCBI Summary for TPP1
This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq, Jul 2008]
UniProt Comments for TPP1
TPP1: Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus. Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2). A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN2 consists of curvilinear profiles. Belongs to the peptidase S53 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; Secreted; Mitochondrial; EC 3.4.14.9; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11p15
Cellular Component: lysosomal lumen; mitochondrion; lysosome; melanosome
Molecular Function: tripeptidyl-peptidase activity; peptidase activity; protein binding; serine-type peptidase activity; metal ion binding; serine-type endopeptidase activity; endopeptidase activity; peptide binding
Biological Process: nervous system development; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; epithelial cell differentiation; lysosome organization and biogenesis; unfolded protein response; peptide catabolic process; protein catabolic process; lipid metabolic process; neuromuscular process controlling balance; proteolysis; bone resorption
Disease: Ceroid Lipofuscinosis, Neuronal, 2
Research Articles on TPP1
1. Mutations have been identified in the TEN-domain of TERT that disrupt the interaction of telomerase with TPP1 in vivo and in vitro but have very little effect on the catalytic activity of telomerase.
Precautions
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Disclaimer
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