Rat 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA kit; CBAS3; CP7B; SPG5A; cytochrome P450; subfamily VIIB (oxysterol 7 alpha-hydroxylase); polypeptide 1; oxysterol 7alpha-hydroxylase; cytochrome P450; family 7; subfamily B; polypeptide 1

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of CYP7B1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9338470 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP7B1. The ELISA analytical biochemical technique of the MBS9338470 kit is based on CYP7B1 antibody-CYP7B1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP7B1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP7B1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

48,227 Da

cytochrome P450, family 7, subfamily b, polypeptide 1

25-hydroxycholesterol 7-alpha-hydroxylase; HCT-1; cytochrome P450 7B1; oxysterol 7-alpha-hydroxylase; hippocampal transcript 1 protein; cytochrome P450, subfamily 7B, polypeptide 1

25-hydroxycholesterol 7-alpha-hydroxylase

HCT-1??[Similar Products]

CP7B1_RAT

member of the p450 subfamily of cholesterol metabolizing enzymes that performs 7alpha-hydroxylation of 27-hydroxycholesterol in a bile synthesis pathway alternative to the classical cholesterol disposal pathway [RGD, Feb 2006]

CYP7B1: Defects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3). Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.13.100; Endoplasmic reticulum; Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase

Cellular Component: endoplasmic reticulum membrane

Molecular Function: 25-hydroxycholesterol 7alpha-hydroxylase activity; iron ion binding; heme binding; oxysterol 7-alpha-hydroxylase activity

Biological Process: response to drug; circadian rhythm; cholesterol metabolic process; bile acid biosynthetic process; response to cAMP; memory; positive regulation of epithelial cell proliferation; negative regulation of estrogen receptor signaling pathway

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