25 hydroxycholesterol 7 alpha hydroxylase; 25-hydroxycholesterol 7-alpha-hydroxylase; CP7B; CP7B1_HUMAN; Cyp7b1; Cytochrome P450 7B1; Cytochrome P450 family 7 subfamily B polypeptide 1; Cytochrome P450 subfamily VIIB polypeptide 1; Oxysterol 7-alpha-hydroxylase; Oxysterol 7alpha hydroxylase

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Cytochrome P450 7B1 antibody detects endogenous levels of total Cytochrome P450 7B1

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1mg/ml (lot specific)

Store at -20 degree C. Stable for 12 months from date of receipt.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Small volumes of anti-CYP7B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Function: Oxysterol 7alpha-hydroxylase that mediates formation of 7-alpha, 25-dihydroxycholesterol (7-alpha, 25-OHC) from 25-hydroxycholesterol (PubMed:10588945). Plays a key role in cell positioning and movement in lymphoid tissues: 7-alpha, 25-dihydroxycholesterol (7-alpha, 25-OHC) acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (By similarity).
Similarity: Belongs to the cytochrome P450 family.

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Western blot analysis of extracts from LOVO cells, using Cytochrome P450 7B1 antibody.The lane on the left is treated with the antigen-specific peptide.

MBS9605048 staining LOVO cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

Observed: 58 kD
Predicted: 59 kDa

cytochrome P450 family 7 subfamily B member 1

25-hydroxycholesterol 7-alpha-hydroxylase

25-hydroxycholesterol 7-alpha-hydroxylase

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016]

Oxysterol 7alpha-hydroxylase that mediates formation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) from 25-hydroxycholesterol (PubMed:10588945). Plays a key role in cell positioning and movement in lymphoid tissues: 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells ().

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