Product Name
CYP7B1, siRNA
Full Product Name
CYP7B1 siRNA (Mouse)
Product Synonym Names
25-hydroxycholesterol 7-alpha-hydroxylase; Cytochrome P450 7B1; Hippocampal transcript 1 protein; HCT-1; Oxysterol 7-alpha-hydroxylase
Product Gene Name
CYP7B1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q60991
Specificity
CYP7B1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CYP7B1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CYP7B1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CYP7B1 sirna
siRNA to inhibit CYP7B1 expression using RNA interference
Applications Tested/Suitable for CYP7B1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CYP7B1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_031851.3
[Other Products]
NCBI GenBank Nucleotide #
NM_007825.4
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UniProt Primary Accession #
Q60991
[Other Products]
UniProt Secondary Accession #
Q9CZ39[Other Products]
UniProt Related Accession #
Q60991[Other Products]
Molecular Weight
58,470 Da
NCBI Official Full Name
25-hydroxycholesterol 7-alpha-hydroxylase
NCBI Official Synonym Full Names
cytochrome P450, family 7, subfamily b, polypeptide 1
NCBI Official Symbol
Cyp7b1??[Similar Products]
NCBI Official Synonym Symbols
hct-1; AW261589; D3Ertd552e
??[Similar Products]
NCBI Protein Information
25-hydroxycholesterol 7-alpha-hydroxylase
UniProt Protein Name
25-hydroxycholesterol 7-alpha-hydroxylase
UniProt Synonym Protein Names
Cytochrome P450 7B1; Hippocampal transcript 1 protein; HCT-1; Oxysterol 7-alpha-hydroxylase
Protein Family
25-hydroxycholesterol 7-alpha-hydroxylase
UniProt Gene Name
Cyp7b1??[Similar Products]
UniProt Synonym Gene Names
HCT-1??[Similar Products]
UniProt Entry Name
CP7B1_MOUSE
UniProt Comments for CYP7B1
CYP7B1: Defects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3). Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable. Belongs to the cytochrome P450 family.
Protein type: Endoplasmic reticulum; EC 1.14.13.100; Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase
Cellular Component: membrane; intracellular membrane-bound organelle; endoplasmic reticulum
Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; 25-hydroxycholesterol 7alpha-hydroxylase activity; metal ion binding; iron ion binding; heme binding; oxidoreductase activity; oxysterol 7-alpha-hydroxylase activity; monooxygenase activity
Biological Process: steroid metabolic process; circadian rhythm; cholesterol metabolic process; bile acid biosynthetic process; digestion; lipid metabolic process; positive regulation of epithelial cell proliferation; memory; negative regulation of estrogen receptor signaling pathway
Research Articles on CYP7B1
1. Findings indicate that CYP7B1 and HSD3B7, as well as CH25H, have essential roles in controlling oxysterol production in lymphoid tissues.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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