Full Product Name
TAC3 siRNA (Human)
Product Synonym Names
NKNB; Tachykinin-3; ZNEUROK1
Product Gene Name
TAC3 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UHF0
Specificity
TAC3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TAC3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TAC3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TAC3 sirna
siRNA to inhibit TAC3 expression using RNA interference
Applications Tested/Suitable for TAC3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TAC3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001171525.1
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NCBI GenBank Nucleotide #
NM_001178054.1
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UniProt Primary Accession #
Q9UHF0
[Other Products]
UniProt Secondary Accession #
Q6IAG2; Q71BC6; Q71BC9[Other Products]
UniProt Related Accession #
Q9UHF0[Other Products]
Molecular Weight
11,358 Da
NCBI Official Full Name
tachykinin-3 isoform 2 preproprotein
NCBI Official Synonym Full Names
tachykinin 3
NCBI Official Symbol
TAC3??[Similar Products]
NCBI Official Synonym Symbols
NKB; HH10; NKNB; PRO1155; ZNEUROK1
??[Similar Products]
NCBI Protein Information
tachykinin-3
UniProt Protein Name
Tachykinin-3
UniProt Synonym Protein Names
ZNEUROK1
Protein Family
Tachykinin
UniProt Gene Name
TAC3??[Similar Products]
UniProt Synonym Gene Names
NKNB; NKB??[Similar Products]
UniProt Entry Name
TKNK_HUMAN
NCBI Summary for TAC3
This gene encodes a member of the tachykinin family of secreted neuropeptides. The encoded protein is primarily expressed in the central and peripheral nervous system and functions as a neurotransmitter. This protein is the ligand for the neurokinin-3 receptor. This protein is also expressed in the outer syncytiotrophoblast of the placenta and may be associated with pregnancy-induced hypertension and pre-eclampsia. Mutations in this gene are associated with normosmic hypogonadotropic hypogonadism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
UniProt Comments for TAC3
TAC3: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Belongs to the tachykinin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; receptor binding
Biological Process: tachykinin signaling pathway; neuropeptide signaling pathway; female pregnancy
Disease: Hypogonadotropic Hypogonadism 10 With Or Without Anosmia
Research Articles on TAC3
1. SP may influence the KP and NKB secretory output via additional autocrine/paracrine mechanisms or regulate GnRH neurosecretion directly.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
