Product Name
Neurokinin B (NKB), Recombinant Protein
Full Product Name
Recombinant Neurokinin B (NKB)
Product Gene Name
NKB recombinant protein
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Matching Pairs
Unconjugated
Antibody: Neurokinin B (MBS2027440)
Immunogen: Neurokinin B (MBS2028998)
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APC-CY7 Conjugated Antibody: Neurokinin B (NKB) (MBS2052467)
Immunogen: Neurokinin B (MBS2028998)
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PE Conjugated Antibody: Neurokinin B (NKB) (MBS2052468)
Immunogen: Neurokinin B (MBS2028998)
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Immunogen: Neurokinin B (MBS2028998)
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Cy3 Conjugated Antibody: Neurokinin B (NKB) (MBS2052470)
Immunogen: Neurokinin B (MBS2028998)
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FITC Conjugated Antibody: Neurokinin B (NKB) (MBS2052471)
Immunogen: Neurokinin B (MBS2028998)
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HRP Conjugated Antibody: Neurokinin B (NKB) (MBS2052472)
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWK I KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-QG GRLSKDSDLY QLPPSLLRRL YDSRPVSLEG LLKVLSKASV GPKETSLPQK RDMHDFFVGL MGKRNSQPDT P
Host
Host: E Coli
Source: Prokaryotic expression
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
7.2 (lot specific)
Predicted Molecular Mass
40.5kDa
Accurate Molecular Mass (KD)
38kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and GST-tag
Organism Species
Mus musculus (Mouse)
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of NKB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for NKB recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
SDS-Page of NKB recombinant protein
NCBI/Uniprot data below describe general gene information for NKB. It may not necessarily be applicable to this product.
NCBI Accession #
AAF76980.1
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UniProt Secondary Accession #
Q6IAG2; Q71BC6; Q71BC9[Other Products]
UniProt Related Accession #
Q9UHF0[Other Products]
Molecular Weight
11,358 Da
NCBI Official Full Name
neurokinin B
NCBI Official Synonym Full Names
tachykinin 3
NCBI Official Symbol
TAC3??[Similar Products]
NCBI Official Synonym Symbols
NKB; HH10; NKNB; PRO1155; ZNEUROK1
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NCBI Protein Information
tachykinin-3
UniProt Protein Name
Tachykinin-3
UniProt Synonym Protein Names
ZNEUROK1
UniProt Gene Name
TAC3??[Similar Products]
UniProt Synonym Gene Names
NKNB; NKB??[Similar Products]
UniProt Entry Name
TKNK_HUMAN
NCBI Summary for NKB
This gene encodes a member of the tachykinin family of secreted neuropeptides. The encoded protein is primarily expressed in the central and peripheral nervous system and functions as a neurotransmitter. This protein is the ligand for the neurokinin-3 receptor. This protein is also expressed in the outer syncytiotrophoblast of the placenta and may be associated with pregnancy-induced hypertension and pre-eclampsia. Mutations in this gene are associated with normosmic hypogonadotropic hypogonadism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
UniProt Comments for NKB
TAC3: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Belongs to the tachykinin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; receptor binding
Biological Process: tachykinin signaling pathway; neuropeptide signaling pathway; female pregnancy
Disease: Hypogonadotropic Hypogonadism 10 With Or Without Anosmia
Research Articles on NKB
1. SP may influence the KP and NKB secretory output via additional autocrine/paracrine mechanisms or regulate GnRH neurosecretion directly.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.