Full Product Name
MSH2 siRNA (Human)
Product Synonym Names
DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2
Product Gene Name
MSH2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P43246
Specificity
MSH2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MSH2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MSH2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MSH2 sirna
siRNA to inhibit MSH2 expression using RNA interference
Applications Tested/Suitable for MSH2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MSH2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000242.1
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NCBI GenBank Nucleotide #
NM_000251.2
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UniProt Primary Accession #
P43246
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UniProt Secondary Accession #
O75488; B4E2Z2[Other Products]
UniProt Related Accession #
P43246[Other Products]
Molecular Weight
97,323 Da
NCBI Official Full Name
DNA mismatch repair protein Msh2 isoform 1
NCBI Official Synonym Full Names
mutS homolog 2
NCBI Official Symbol
MSH2??[Similar Products]
NCBI Official Synonym Symbols
FCC1; COCA1; HNPCC; LCFS2; HNPCC1
??[Similar Products]
NCBI Protein Information
DNA mismatch repair protein Msh2
UniProt Protein Name
DNA mismatch repair protein Msh2
UniProt Synonym Protein Names
MutS protein homolog 2
Protein Family
DNA mismatch repair protein
UniProt Gene Name
MSH2??[Similar Products]
UniProt Synonym Gene Names
hMSH2??[Similar Products]
UniProt Entry Name
MSH2_HUMAN
NCBI Summary for MSH2
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Comments for MSH2
MSH2: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. Heterodimer consisting of MSH2-MSH6 (MutS alpha) or MSH2- MSH3 (MutS beta). Both heterodimer form a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Interacts with SLX4/BTBD12; this interaction is direct and links MutS beta to SLX4, a subunit of different structure-specific endonucleases. Interacts with SMARCAD1. Ubiquitously expressed. Belongs to the DNA mismatch repair MutS family.
Protein type: Tumor suppressor; DNA-binding
Chromosomal Location of Human Ortholog: 2p21
Cellular Component: nucleoplasm; nuclear chromosome; membrane; MutSalpha complex; MutSbeta complex
Molecular Function: protein homodimerization activity; dinucleotide repeat insertion binding; single thymine insertion binding; ATPase activity; magnesium ion binding; loop DNA binding; Y-form DNA binding; enzyme binding; four-way junction DNA binding; single guanine insertion binding; double-stranded DNA binding; guanine/thymine mispair binding; ATP binding; single-stranded DNA binding; protein C-terminus binding; DNA-dependent ATPase activity; double-strand/single-strand DNA junction binding; oxidized purine DNA binding; ADP binding; protein kinase binding; mismatched DNA binding; centromeric DNA binding; protein binding; DNA binding; MutLalpha complex binding; dinucleotide insertion or deletion binding
Biological Process: determination of ***** life span; maintenance of DNA repeat elements; germ cell development; positive regulation of helicase activity; double-strand break repair; negative regulation of neuron apoptosis; cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to X-ray; oxidative phosphorylation; negative regulation of DNA recombination; mismatch repair; in utero embryonic development; postreplication repair; somatic hypermutation of immunoglobulin genes; male gonad development; isotype switching; DNA repair; response to UV-B; meiotic mismatch repair; B cell mediated immunity; intra-S DNA damage checkpoint; B cell differentiation; somatic recombination of immunoglobulin gene segments; meiotic gene conversion; negative regulation of meiotic recombination
Disease: Muir-torre Syndrome; Mismatch Repair Cancer Syndrome; Lynch Syndrome I
Research Articles on MSH2
1. A novel mutation consisting in a G deletion at 914 codon of the exon 16 in the MSH2 gene that is associated with HNPCC is described.
Precautions
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