For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

X75

Mouse

This item recognises the ovarian cancer mucin CA125 (also known as MUCIN 16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces.

CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. This item recognises the epitope specificity group B, similar to the M-11 antibody.

Purified
Purified IgG - liquid

IgG concentration 1.0mg/ml (lot specific)

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: 18 months from date of despatch.

Small volumes of anti-CA125 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse anti Human CA125 antibody, clone X75 recognizes the ovarian cancer mucin CA125 (also known as MUCIN 16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M-11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces. CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Mouse anti Human CA125 antibody, clone X75 recognizes the epitope specificity group B, similar to the M-11 antibody (Nustad et al. 2002). Mouse anti Human CA125 antibody, clone X75 has been used sucessfully as both a capture and detection reagent in the development of sensitive ELISA assays for the measurement of CA125 antigen in human plasma samples.

ELISA (EIA), Western Blot (WB)

Sandwich ELISA analysis of human CA-125 expression using Mouse anti Human CA-125 antibody, clone X75 (MBS214596) as a capture reagent and biotinylated Mouse anti Human CA-125 antibody, clone X325 (MBS214597) as a detection reagent with purified human CA-125 as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma (green) sample at 1:2 is shown

Sandwich ELISA analysis of human CA-125 expression using Mouse anti Human CA-125 antibody, clone X75 as a capture reagent and biotinylated Mouse anti Human CA-125 antibody, clone X325 (MBS214596) as a detection reagent with purified human CA-125 as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma (green) sample at 1:2 is shown

2,353,428 Da

mucin 16, cell surface associated

mucin-16; CA125 ovarian cancer antigen; ovarian cancer-related tumor marker CA125; ovarian carcinoma antigen CA125

Mucin-16

CA125; MUC-16; CA-125??[Similar Products]

MUC16_HUMAN

MUC16: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

Protein type: Cell adhesion; Membrane protein, peripheral; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; extrinsic to membrane; Golgi lumen; integral to membrane; plasma membrane; vesicle

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; cell adhesion; post-translational protein modification

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