Full Product Name
MSH2 Antibody
Product Synonym Names
COCA1; DNA mismatch repair protein Msh2; FCC1; HNPCC; HNPCC1; MutS protein homolog 2; mutS (E. coli) homolog 2
Product Gene Name
anti-MSH2 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P43246
Species Reactivity
Human, Mouse, Rat
Specificity
MSH2 Antibody detects endogenous levels of total MSH2 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human MSH2.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-MSH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MSH2 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-MSH2 antibody
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000
Immunofluorescence (IF) of anti-MSH2 antibody
Immunofluorescence analysis of HUVEC cells, using MSH2 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry (IHC) of anti-MSH2 antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MSH2 Antibody. The picture on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for MSH2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000242.1
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NCBI GenBank Nucleotide #
NM_000251.2
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UniProt Primary Accession #
P43246
[Other Products]
UniProt Secondary Accession #
O75488; B4E2Z2[Other Products]
UniProt Related Accession #
P43246[Other Products]
NCBI Official Full Name
DNA mismatch repair protein Msh2 isoform 1
NCBI Official Synonym Full Names
mutS homolog 2
NCBI Official Symbol
MSH2??[Similar Products]
NCBI Official Synonym Symbols
FCC1; COCA1; HNPCC; LCFS2; HNPCC1
??[Similar Products]
NCBI Protein Information
DNA mismatch repair protein Msh2
UniProt Protein Name
DNA mismatch repair protein Msh2
UniProt Synonym Protein Names
MutS protein homolog 2
Protein Family
DNA mismatch repair protein
UniProt Gene Name
MSH2??[Similar Products]
UniProt Synonym Gene Names
hMSH2??[Similar Products]
NCBI Summary for MSH2
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Comments for MSH2
Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Research Articles on MSH2
1. Loss of MSH2 expression is associated with colorectal carcinoma.
Precautions
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Disclaimer
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