For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of API4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7253341 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apoptosis inhibitor 4 (API4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing API4. The ELISA analytical biochemical technique of the MBS7253341 kit is based on API4 antibody-API4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect API4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, API4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Cell Biology

8,490 Da

baculoviral IAP repeat containing 5

baculoviral IAP repeat-containing protein 5; apoptosis inhibitor 4; survivin variant 3 alpha; apoptosis inhibitor survivin

Baculoviral IAP repeat-containing protein 5

API4; IAP4??[Similar Products]

BIRC5_HUMAN

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in ***** tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

Survivin: an apoptosis inhibitor that is expressed during the G2/M phase of the cell cycle. Associates with the microtubules of the mitotic spindle and any disruption results in the loss of apoptosis activity. May play a role in neoplasia. Inhibitor of caspase-3 and caspase-7. Two splice variant isoforms have been described.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: centriole; nuclear chromosome; microtubule; cytoplasmic microtubule; spindle microtubule; cytoplasm; spindle; midbody; nucleus; cytosol; interphase microtubule organizing center; chromosome, pericentric region

Molecular Function: tubulin binding; identical protein binding; protein homodimerization activity; zinc ion binding; microtubule binding; metal ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; protein binding; enzyme binding; protein heterodimerization activity; chaperone binding; cysteine protease inhibitor activity; cofactor binding; Ran GTPase binding; cobalt ion binding

Biological Process: mitosis; transcription, DNA-dependent; spindle checkpoint; establishment of chromosome localization; protein ubiquitination; cytokinesis; regulation of signal transduction; negative regulation of caspase activity; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation; protein complex localization; cell division; positive regulation of cell proliferation; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G2/M transition of mitotic cell cycle; positive regulation of exit from mitosis; negative regulation of apoptosis

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