Product Name
PHOSPHORYLASE B (PHKB), Polyclonal Antibody
Full Product Name
SHEEP ANTI RABBIT PHOSPHORYLASE B
Product Gene Name
anti-PHKB antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P12798
Specificity
High cross reactivity (95-135%) with Phosphorylase a in ELISA.
Form/Format
Serum
Serum - liquid
Immunogen
Native, from muscle
Preparation and Storage
Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-PHKB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PHKB antibody
MBS223707 recognises phosphorylase B, a catalytic protein with a predicted molecular weight of 125 kDa localized in the cytoplasm and cell membrane. In skeletal muscle, phosphorylase occurs in two forms, phosphorylase A and phosphorylase B. Phosphorylase A is the more active of the two forms and has a higher molecular weight. The two forms are interconvertable. Phosphorylase B catalyzes the phosphorylation of serine residues in various substrates including troponin I. MBS223707 shows high cross reactivity (>95%) with phosphorylase A in ELISA.
Applications Tested/Suitable for anti-PHKB antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-PHKB antibody
ELISA: Minimum Dilution: 1/600; Maximum Dilution: 1/2000;
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/2000
Testing Data #1 of anti-PHKB antibody
Rabbit Phosphorylase B detected with Sheep anti Rabbit Phosphorylase B (MBS223707)
NCBI/Uniprot data below describe general gene information for PHKB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001075770.1
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NCBI GenBank Nucleotide #
NM_001082301.1
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UniProt Primary Accession #
P12798
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UniProt Related Accession #
P12798[Other Products]
Molecular Weight
124,170 Da[Similar Products]
NCBI Official Full Name
phosphorylase b kinase regulatory subunit beta
NCBI Official Symbol
PHKB??[Similar Products]
NCBI Protein Information
phosphorylase b kinase regulatory subunit beta; phosphorylase kinase beta-subunit; phosphorylase kinase subunit beta
UniProt Protein Name
Phosphorylase b kinase regulatory subunit beta
Protein Family
Phosphorylase b kinase
UniProt Gene Name
PHKB??[Similar Products]
UniProt Synonym Gene Names
Phosphorylase kinase subunit beta??[Similar Products]
UniProt Entry Name
KPBB_RABIT
UniProt Comments for PHKB
PHKB: Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation. Defects in PHKB are the cause of glycogen storage disease type 9B (GSD9B); also known as phosphorylase kinase deficiency of liver and muscle (PKD). GSD9B is a metabolic disorder characterized by hepathomegaly, only slightly elevated transaminases and plasma lipids, clinical improvement with increasing age, and remarkably no clinical muscle involvement. Biochemical observations suggest that this mild phenotype is caused by an incomplete holoenzyme that lacks the beta subunit, but that may possess residual activity. Belongs to the phosphorylase b kinase regulatory chain family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, regulatory subunit
Research Articles on PHKB
1. Interaction of the wild type (wt) heat shock protein Hsp27 and its three-dimensional (3D) mutant (mimicking phosphorylation at Ser15, 78, and 82) with rabbit skeletal muscle phosphorylase kinase (PhK) has been studied under crowding conditions.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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