Full Product Name
PHKB siRNA (Mouse)
Product Synonym Names
Phosphorylase b kinase regulatory subunit beta; Phosphorylase kinase subunit beta
Product Gene Name
PHKB sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q7TSH2
Specificity
PHKB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PHKB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PHKB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PHKB sirna
siRNA to inhibit PHKB expression using RNA interference
Applications Tested/Suitable for PHKB sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PHKB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_955517.1
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NCBI GenBank Nucleotide #
NM_199446.1
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UniProt Primary Accession #
Q7TSH2
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UniProt Related Accession #
Q7TSH2[Other Products]
Molecular Weight
123,889 Da
NCBI Official Full Name
phosphorylase b kinase regulatory subunit beta
NCBI Official Synonym Full Names
phosphorylase kinase beta
NCBI Official Symbol
Phkb??[Similar Products]
NCBI Official Synonym Symbols
AI463271
??[Similar Products]
NCBI Protein Information
phosphorylase b kinase regulatory subunit beta
UniProt Protein Name
Phosphorylase b kinase regulatory subunit beta
Protein Family
Phosphorylase b kinase regulatory
UniProt Gene Name
Phkb??[Similar Products]
UniProt Synonym Gene Names
Phosphorylase kinase subunit beta??[Similar Products]
UniProt Entry Name
KPBB_MOUSE
UniProt Comments for PHKB
PHKB: Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation. Defects in PHKB are the cause of glycogen storage disease type 9B (GSD9B); also known as phosphorylase kinase deficiency of liver and muscle (PKD). GSD9B is a metabolic disorder characterized by hepathomegaly, only slightly elevated transaminases and plasma lipids, clinical improvement with increasing age, and remarkably no clinical muscle involvement. Biochemical observations suggest that this mild phenotype is caused by an incomplete holoenzyme that lacks the beta subunit, but that may possess residual activity. Belongs to the phosphorylase b kinase regulatory chain family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, regulatory subunit
Cellular Component: membrane; plasma membrane; phosphorylase kinase complex
Molecular Function: calmodulin binding; phosphorylase kinase activity; catalytic activity; hydrolase activity, hydrolyzing O-glycosyl compounds
Biological Process: glycogen metabolic process; carbohydrate metabolic process; polysaccharide metabolic process; protein amino acid phosphorylation
Research Articles on PHKB
1. The interactions described herein between the beta subunit of PhK and GAPDH provide a possible mechanism for the direct linkage of glycogenolysis and glycolysis in skeletal muscle.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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