Product Name
KIR3DS1, siRNA
Full Product Name
KIR3DS1 siRNA (Human)
Product Synonym Names
NKAT10; Killer cell immunoglobulin-like receptor 3DS1; MHC class I NK cell receptor; Natural killer-associated transcript 10; NKAT-10
Product Gene Name
KIR3DS1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q14943
Specificity
KIR3DS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KIR3DS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of KIR3DS1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KIR3DS1 sirna
siRNA to inhibit KIR3DS1 expression using RNA interference
Applications Tested/Suitable for KIR3DS1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for KIR3DS1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001077008.1
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NCBI GenBank Nucleotide #
NM_001083539.2
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UniProt Primary Accession #
Q14943
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UniProt Related Accession #
Q14943[Other Products]
Molecular Weight
42,902 Da
NCBI Official Full Name
killer cell immunoglobulin-like receptor 3DS1 isoform 1
NCBI Official Synonym Full Names
killer cell immunoglobulin-like receptor, three domains, short cytoplasmic tail, 1
NCBI Official Symbol
KIR3DS1??[Similar Products]
NCBI Official Synonym Symbols
KIR-G1; NKAT10; CD158E2; NKAT-10; KIR-123FM
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NCBI Protein Information
killer cell immunoglobulin-like receptor 3DS1
UniProt Protein Name
Killer cell immunoglobulin-like receptor 3DS1
UniProt Synonym Protein Names
MHC class I NK cell receptor; Natural killer-associated transcript 10; NKAT-10
Protein Family
Killer cell immunoglobulin-like receptor
UniProt Gene Name
KIR3DS1??[Similar Products]
UniProt Synonym Gene Names
NKAT10; NKAT-10??[Similar Products]
UniProt Entry Name
KI3S1_HUMAN
NCBI Summary for KIR3DS1
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]
UniProt Comments for KIR3DS1
KIR3DS1: Receptor on natural killer (NK) cells for HLA-C alleles. Does not inhibit the activity of NK cells. Belongs to the immunoglobulin superfamily.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.4
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: MHC class I receptor activity
Biological Process: innate immune response; natural killer cell activation; immune response; signal transduction
Research Articles on KIR3DS1
1. Data show that KIR2DL5 receptor, KIR2DS1 protein, KIR2DS5 protein and KIR3DS1 receptors were all significantly associated with high viral load.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
