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apoptosis inhibitor 4 (API4), ELISA Kit

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產(chǎn)品名稱: apoptosis inhibitor 4 (API4), ELISA Kit
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apoptosis inhibitor 4 (API4), ELISA Kit


apoptosis inhibitor 4 (API4), ELISA Kit  的詳細介紹
Product Name

apoptosis inhibitor 4 (API4) (BIRC5), ELISA Kit

Full Product Name

Canine apoptosis inhibitor 4 (API4) ELISA Kit

Product Gene Name

BIRC5 elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Request for Current Manual Insert
Request Current Manual
OMIM
603352
3D Structure
ModBase 3D Structure for O15392
Species Reactivity
Canine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of BIRC5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for BIRC5 purchase
MBS7232824 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apoptosis inhibitor 4 (API4) (BIRC5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BIRC5. The ELISA analytical biochemical technique of the MBS7232824 kit is based on BIRC5 antibody-BIRC5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BIRC5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BIRC5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Categories/Family for BIRC5 elisa kit
Cell Biology
NCBI/Uniprot data below describe general gene information for BIRC5. It may not necessarily be applicable to this product.
NCBI GI #
49456439
NCBI GeneID
332
NCBI Accession #
CAG46540.1 [Other Products]
UniProt Primary Accession #
O15392 [Other Products]
UniProt Secondary Accession #
Q2I3N8; Q4VGX0; Q53F61; Q5MGC6; Q6FHL2; Q75SP2; Q9P2W8; A2SUH6; B2R4R1[Other Products]
UniProt Related Accession #
O15392[Other Products]
Molecular Weight
8,490 Da
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NCBI Official Full Name
BIRC5
NCBI Official Synonym Full Names
baculoviral IAP repeat containing 5
NCBI Official Symbol
BIRC5??[Similar Products]
NCBI Official Synonym Symbols
API4; EPR-1
??[Similar Products]
NCBI Protein Information
baculoviral IAP repeat-containing protein 5; apoptosis inhibitor 4; survivin variant 3 alpha; apoptosis inhibitor survivin
UniProt Protein Name
Baculoviral IAP repeat-containing protein 5
UniProt Synonym Protein Names
Apoptosis inhibitor 4; Apoptosis inhibitor survivin
Protein Family
Baculoviral IAP repeat-containing protein
UniProt Gene Name
BIRC5??[Similar Products]
UniProt Synonym Gene Names
API4; IAP4??[Similar Products]
UniProt Entry Name
BIRC5_HUMAN
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NCBI Summary for BIRC5
This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in ***** tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
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UniProt Comments for BIRC5
Survivin: an apoptosis inhibitor that is expressed during the G2/M phase of the cell cycle. Associates with the microtubules of the mitotic spindle and any disruption results in the loss of apoptosis activity. May play a role in neoplasia. Inhibitor of caspase-3 and caspase-7. Two splice variant isoforms have been described.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: centriole; nuclear chromosome; microtubule; cytoplasmic microtubule; spindle microtubule; cytoplasm; spindle; midbody; nucleus; interphase microtubule organizing center; cytosol; chromosome, pericentric region

Molecular Function: tubulin binding; identical protein binding; protein homodimerization activity; zinc ion binding; metal ion binding; microtubule binding; caspase inhibitor activity; ubiquitin-protein ligase activity; protein binding; enzyme binding; protein heterodimerization activity; chaperone binding; cofactor binding; cysteine protease inhibitor activity; Ran GTPase binding; cobalt ion binding

Biological Process: mitosis; transcription, DNA-dependent; spindle checkpoint; establishment of chromosome localization; regulation of signal transduction; protein ubiquitination; cytokinesis; negative regulation of caspase activity; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation; protein complex localization; cell division; positive regulation of cell proliferation; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent; positive regulation of exit from mitosis; negative regulation of apoptosis
Research Articles on BIRC5
1. siRNA-mediated silencing of survivin inhibits proliferation and enhances etoposide chemosensitivity in acute myeloid leukemia.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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