AKR1C1/Aldo-keto reductase family 1 member C1/Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase/Indanol dehydrogenase/High-affinity hepatic bile acid-binding protein/HBAB/Dihydrodiol dehydrogenase 1/2/DD1/DD2/20-alpha-hydroxysteroid dehydrogenase/20-alpha-HSD/Chlordecone reductase homolog HAKRC/DDH/DDH1/C9/H-37/MBAB/2-ALPHA-HSD

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of AKR1C1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS762247 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aldo-keto reductase family 1 member C1 (AKR1C1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AKR1C1. The ELISA analytical biochemical technique of the MBS762247 kit is based on AKR1C1 antibody-AKR1C1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AKR1C1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AKR1C1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human AKR1C1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human AKR1C1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human AKR1C1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman AKR1C1can be calculated.

36,788 Da

aldo-keto reductase family 1, member C1

aldo-keto reductase family 1 member C1

Aldo-keto reductase family 1 member C1

DDH; DDH1; 20-alpha-HSD; DD1/DD2; HBAB??[Similar Products]

AK1C1_HUMAN

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

AKR1C1: Converts progesterone to its inactive form, 20-alpha- dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation. Belongs to the aldo/keto reductase family.

Protein type: Oxidoreductase; EC 1.3.1.20; EC 1.1.1.149; EC 1.1.1.112; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytosol

Molecular Function: 20-alpha-hydroxysteroid dehydrogenase activity; 3-alpha-hydroxysteroid dehydrogenase (B-specific) activity; aldehyde reductase activity; aldo-keto reductase activity; bile acid binding; carboxylic acid binding; indanol dehydrogenase activity; ketosteroid monooxygenase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; phenanthrene 9,10-monooxygenase activity; protein binding; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity

Biological Process: bile acid and bile salt transport; bile acid metabolic process; cholesterol absorption; cholesterol homeostasis; digestion; epithelial cell differentiation; fat-soluble vitamin metabolic process; phototransduction, visible light; progesterone metabolic process; protein homooligomerization; response to organophosphorus; retinal metabolic process; retinoid metabolic process; vitamin metabolic process; xenobiotic metabolic process

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