Aldo-keto reductase family 1 member C2; 1---; 3-alpha-HSD3; Chlordecone reductase homolog HAKRD; Dihydrodiol dehydrogenase 2; DD-2; DD2; Dihydrodiol dehydrogenase/bile acid-binding protein; DD/BABP; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; Type III 3-alpha-hydroxysteroid dehydrogenase; AKR1C2; DDH2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This AKR1C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-323 amino acids from the C-terminal region of human AKR1C2.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.45 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-AKR1C2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the aldo/keto reductase
superfamily, which consists of more than 40 known enzymes and
proteins. These enzymes catalyze the conversion of aldehydes and
ketones to their corresponding alcohols using NADH and/or NADPH as
cofactors. The enzymes display overlapping but distinct substrate
specificity. This enzyme binds bile acid with high affinity, and
shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This
gene shares high sequence identity with three other gene members
and is clustered with those three genes at chromosome 10p15-p14.

Cancer; Developmental Biology; Neuroscience; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

WB~~1:1000

AKR1C2 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the AKR1C2 antibody detected the AKR1C2 protein (arrow).

AKR1C2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKR1C2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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aldo-keto reductase family 1, member C2

aldo-keto reductase family 1 member C2

Aldo-keto reductase family 1 member C2

DDH2; DD-2; DD2; DD/BABP??[Similar Products]

AK1C2_HUMAN

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

AKR1C2: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. Defects in AKR1C2 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. Belongs to the aldo/keto reductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.357; Oxidoreductase; EC 1.3.1.20; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; bile acid binding; carboxylic acid binding; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity

Biological Process: steroid metabolic process; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; epithelial cell differentiation; digestion; positive regulation of cell proliferation; progesterone metabolic process; prostaglandin metabolic process

Disease: 46,xy Sex Reversal 8

Setlur, S.R., et al. Cancer Epidemiol. Biomarkers Prev. 19(1):229-239(2010)
Wang, X., et al. PLoS ONE 5 (8), E11934 (2010) :
Reding, K.W., et al. Am. J. Epidemiol. 170(10):1241-1249(2009)
Cogliati, C., et al. FEBS J. 276(20):6011-6023(2009)
Davies, N.J., et al. Cancer Res. 69(11):4769-4775(2009)

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