Product Name
AKR1C2, siRNA
Full Product Name
AKR1C2 siRNA (Human)
Product Synonym Names
DDH2; Aldo-keto reductase family 1 member C2; 3-alpha-HSD3; Chlordecone reductase homolog HAKRD; Dihydrodiol dehydrogenase 2; DD-2; DD2; Dihydrodiol dehydrogenase/bile acid-binding protein; DD/BABP; Trans-1.2-dihydrobenzene-1.2-diol dehydrogenase; Type III 3-alpha-hydroxysteroid dehydrogenase
Product Gene Name
AKR1C2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P52895
Specificity
AKR1C2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AKR1C2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AKR1C2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AKR1C2 sirna
siRNA to inhibit AKR1C2 expression using RNA interference
Applications Tested/Suitable for AKR1C2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AKR1C2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001128713.1
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NCBI GenBank Nucleotide #
NM_001135241.2
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UniProt Primary Accession #
P52895
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UniProt Secondary Accession #
Q14133; Q5SR16; Q7M4N1; Q96A71; A8K2N9; B4DKR9[Other Products]
UniProt Related Accession #
P52895[Other Products]
Molecular Weight
15,748 Da
NCBI Official Full Name
aldo-keto reductase family 1 member C2 isoform 2
NCBI Official Synonym Full Names
aldo-keto reductase family 1, member C2
NCBI Official Symbol
AKR1C2??[Similar Products]
NCBI Official Synonym Symbols
DD; DD2; TDD; BABP; DD-2; DDH2; HBAB; HAKRD; MCDR2; SRXY8; DD/BABP; AKR1C-pseudo
??[Similar Products]
NCBI Protein Information
aldo-keto reductase family 1 member C2
UniProt Protein Name
Aldo-keto reductase family 1 member C2
UniProt Synonym Protein Names
3-alpha-HSD3; Chlordecone reductase homolog HAKRD; Dihydrodiol dehydrogenase 2; DD-2; DD2; Dihydrodiol dehydrogenase/bile acid-binding protein; DD/BABP; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (EC:1.3.1.20); Type III 3-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.357)
Protein Family
Aldo-keto reductase family
UniProt Gene Name
AKR1C2??[Similar Products]
UniProt Synonym Gene Names
DDH2; DD-2; DD2; DD/BABP??[Similar Products]
UniProt Entry Name
AK1C2_HUMAN
NCBI Summary for AKR1C2
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Comments for AKR1C2
AKR1C2: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. Defects in AKR1C2 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. Belongs to the aldo/keto reductase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; EC 1.3.1.20; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.357
Chromosomal Location of Human Ortholog: 10p15-p14
Cellular Component: cytoplasm
Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; bile acid binding; carboxylic acid binding; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity
Biological Process: steroid metabolic process; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; epithelial cell differentiation; positive regulation of cell proliferation; digestion; progesterone metabolic process; prostaglandin metabolic process
Disease: 46,xy Sex Reversal 8
Research Articles on AKR1C2
1. In model cell lines of endometrial cancer, AKR1C2 and SRD5A1 have crucial roles in progesterone metabolism.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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