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SLC25A19, siRNA

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產(chǎn)品名稱: SLC25A19, siRNA
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SLC25A19, siRNA


SLC25A19, siRNA  的詳細(xì)介紹
Product Name

SLC25A19, siRNA

Full Product Name

SLC25A19 siRNA (Mouse)

Product Synonym Names
Mitochondrial thiamine pyrophosphate carrier; Solute carrier family 25 member 19
Product Gene Name

SLC25A19 sirna

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for Q9DAM5
Host
Synthetic
Species Reactivity
Mouse
Specificity
SLC25A19 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SLC25A19 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SLC25A19 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
SLC25A19 sirna
siRNA to inhibit SLC25A19 expression using RNA interference
Applications Tested/Suitable for SLC25A19 sirna
RNA Interference (RNAi)
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NCBI/Uniprot data below describe general gene information for SLC25A19. It may not necessarily be applicable to this product.
NCBI GI #
356991183
NCBI GeneID
67283
NCBI Accession #
NP_001239313.1 [Other Products]
NCBI GenBank Nucleotide #
NM_001252384.1 [Other Products]
UniProt Primary Accession #
Q9DAM5 [Other Products]
UniProt Secondary Accession #
Q3T9C4; A2A9V6[Other Products]
UniProt Related Accession #
Q9DAM5[Other Products]
Molecular Weight
35,618 Da
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NCBI Official Full Name
mitochondrial thiamine pyrophosphate carrier isoform 1
NCBI Official Synonym Full Names
solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19
NCBI Official Symbol
Slc25a19??[Similar Products]
NCBI Official Synonym Symbols
DNC; TPC; MUP1; 2900089E13Rik
??[Similar Products]
NCBI Protein Information
mitochondrial thiamine pyrophosphate carrier
UniProt Protein Name
Mitochondrial thiamine pyrophosphate carrier
UniProt Synonym Protein Names
Solute carrier family 25 member 19
Protein Family
Mitochondrial thiamine pyrophosphate carrier
UniProt Gene Name
Slc25a19??[Similar Products]
UniProt Entry Name
TPC_MOUSE
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UniProt Comments for SLC25A19
SLC25A19: Mitochondrial transporter mediating uptake of thiamine pyrophosphate (ThPP) into mitochondria. Defects in SLC25A19 are the cause of microcephaly Amish type (MCPHA); also known as Amish lethal microcephaly. MCPHA is an autosomal recessive metabolic disorder characterized by severe congenital microcephaly, severe 2- ketoglutaric aciduria and death within the first year. Defects in SLC25A19 are the cause of striatal necrosis bilateral and progressive polyneuropathy (SNBPP). A disease characterized by recurrent episodes of flaccid paralysis and encephalopathy associated with bilateral striatal necrosis and chronic progressive polyneuropathy. Belongs to the mitochondrial carrier family.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Mitochondrial

Cellular Component: membrane; mitochondrion; mitochondrial inner membrane; integral to membrane; nucleus

Biological Process: transport; transmembrane transport
Research Articles on SLC25A19
1. We review the evidence that the function of the SLC25A19 gene product, previously identified as the mitochondrial deoxyribonucleotide carrier (DNC), is actually the transport of thiamine pyrophosphate.[review]
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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