Product Name
ARHGEF6, siRNA
Full Product Name
ARHGEF6 siRNA (Rat)
Product Synonym Names
Rho guanine nucleotide exchange factor 6; Rac/Cdc42 guanine nucleotide exchange factor 6
Product Gene Name
ARHGEF6 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q5XXR3
Specificity
ARHGEF6 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ARHGEF6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ARHGEF6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ARHGEF6 sirna
siRNA to inhibit ARHGEF6 expression using RNA interference
Applications Tested/Suitable for ARHGEF6 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ARHGEF6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001005565.1
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NCBI GenBank Nucleotide #
NM_001005565.1
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UniProt Primary Accession #
Q5XXR3
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UniProt Related Accession #
Q5XXR3[Other Products]
Molecular Weight
87,013 Da
NCBI Official Full Name
rho guanine nucleotide exchange factor 6
NCBI Official Synonym Full Names
Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
NCBI Official Symbol
Arhgef6??[Similar Products]
NCBI Protein Information
rho guanine nucleotide exchange factor 6
UniProt Protein Name
Rho guanine nucleotide exchange factor 6
UniProt Synonym Protein Names
Rac/Cdc42 guanine nucleotide exchange factor 6
Protein Family
Rho guanine nucleotide exchange factor
UniProt Gene Name
Arhgef6??[Similar Products]
UniProt Entry Name
ARHG6_RAT
UniProt Comments for ARHGEF6
ARHGEF6: Acts as a RAC1 guanine nucleotide exchange factor (GEF). Interacts with PAK kinases through the SH3 domain. Interacts with GIT1. Component of cytoplasmic complexes, which also contain PXN, GIT1 and PAK1. Ubiquitous. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: GEFs, Rac/Rho; Motility/polarity/chemotaxis; GEFs
Cellular Component: lamellipodium; intercellular junction
Molecular Function: Rho guanyl-nucleotide exchange factor activity
Biological Process: lamellipodium biogenesis
Research Articles on ARHGEF6
1. c-Cbl negatively regulates alphaPix-mediated cell migration and invasion; the lack of c-Cbl in C6 and A172 glioma cells is responsible for their malignant behavior
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
