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OXCT1, Polyclonal Antibody

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OXCT1, Polyclonal Antibody


OXCT1, Polyclonal Antibody  的詳細介紹
Product Name

OXCT1, Polyclonal Antibody

Popular Item
Full Product Name

OXCT1 Antibody

Product Synonym Names
3 oxoacid CoA transferase 1; 3-oxoacid CoA-transferase 1; EC 2.8.3.5; OTTHUMP00000120012; OTTHUMP00000221550; OXCT; Oxct1; SCOT; Scot S; Scot-S; SCOT1_HUMAN; Somatic type succinyl CoA:3 oxoacid CoA transferase; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; Succinyl CoA:3 ketoacid CoA transferase; Succinyl CoA:3 ketoacid coenzyme A transferase 1 mitochondrial; Succinyl CoA:3 oxoacid CoA transferase; Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
Product Gene Name

anti-OXCT1 antibody

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for P55809
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
OXCT1 antibody detects endogenous levels of OXCT1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human OXCT1.
Subcellular Location
Mitochondrion Matrix.
Tissue Specificity
Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (92%), Sheep (92%), Rabbit (100%), Dog (100%), Xenopus (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-OXCT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
anti-OXCT1 antibody
Function: Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Subunit Structure: Homodimer.
Similarity: Belongs to the 3-oxoacid CoA-transferase family.
Applications Tested/Suitable for anti-OXCT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-OXCT1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-OXCT1 antibody
Western blot analysis of extracts from various samples, using OXCT1 antibody. Lane 1: rat brain treated with blocking peptide. Lane 2: rat brain; Lane 3: 293; Lane 4: mouse spleen; Lane 5: hela;
anti-OXCT1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-OXCT1 antibody
MBS9603310 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-OXCT1 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-OXCT1 antibody
Western blot analysis of extracts of human brain tissue, using OXCT1 antibody.
anti-OXCT1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for OXCT1. It may not necessarily be applicable to this product.
NCBI GI #
4557817
NCBI GeneID
5019
NCBI Accession #
NP_000427.1 [Other Products]
NCBI GenBank Nucleotide #
NM_000436.3 [Other Products]
UniProt Primary Accession #
P55809 [Other Products]
UniProt Secondary Accession #
B2R5V2; B7Z528[Other Products]
UniProt Related Accession #
P55809[Other Products]
Molecular Weight
Observed: 56 kDa
Predicted: 57 kDa
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NCBI Official Full Name
succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial isoform 3
NCBI Official Synonym Full Names
3-oxoacid CoA-transferase 1
NCBI Official Symbol
OXCT1??[Similar Products]
NCBI Official Synonym Symbols
OXCT; SCOT
??[Similar Products]
NCBI Protein Information
succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
UniProt Protein Name
Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
UniProt Synonym Protein Names
3-oxoacid CoA-transferase 1; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; SCOT-s
Protein Family
Succinyl-CoA:3-ketoacid coenzyme A transferase
UniProt Gene Name
OXCT1??[Similar Products]
UniProt Synonym Gene Names
OXCT; SCOT; SCOT-s??[Similar Products]
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NCBI Summary for OXCT1
This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq, Jul 2008]
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UniProt Comments for OXCT1
Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Research Articles on OXCT1
1. Crystal structure of human SCOT, providing a molecular understanding of the reported mutations based on their potential structural effects.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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