3 oxoacid CoA transferase 1; 3-oxoacid CoA-transferase 1; EC 2.8.3.5; OTTHUMP00000120012; OTTHUMP00000221550; OXCT; Oxct1; SCOT; Scot S; Scot-S; SCOT1_HUMAN; Somatic type succinyl CoA:3 oxoacid CoA transferase; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; Succinyl CoA:3 ketoacid CoA transferase; Succinyl CoA:3 ketoacid coenzyme A transferase 1 mitochondrial; Succinyl CoA:3 oxoacid CoA transferase; Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

OXCT1 antibody detects endogenous levels of OXCT1.

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1mg/ml (lot specific)

Store at -20 degree C. Stable for 12 months from date of receipt.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Small volumes of anti-OXCT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Function: Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Subunit Structure: Homodimer.
Similarity: Belongs to the 3-oxoacid CoA-transferase family.

Western Blot (WB), Immunohistochemistry (IHC)

WB: 1:500-1:2000
IHC: 1:50-1:200

Western blot analysis of extracts from various samples, using OXCT1 antibody. Lane 1: rat brain treated with blocking peptide. Lane 2: rat brain; Lane 3: 293; Lane 4: mouse spleen; Lane 5: hela;

MBS9603310 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Western blot analysis of extracts of human brain tissue, using OXCT1 antibody.

Observed: 56 kDa
Predicted: 57 kDa

3-oxoacid CoA-transferase 1

succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial

Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial

OXCT; SCOT; SCOT-s??[Similar Products]

This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq, Jul 2008]

Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.

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