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DNA mismatch repair protein Mlh1 (Mlh1), Recombinant Protein

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產(chǎn)品名稱: DNA mismatch repair protein Mlh1 (Mlh1), Recombinant Protein
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DNA mismatch repair protein Mlh1 (Mlh1), Recombinant Protein


DNA mismatch repair protein Mlh1 (Mlh1), Recombinant Protein  的詳細(xì)介紹
Product Name

DNA mismatch repair protein Mlh1 (Mlh1), Recombinant Protein

Full Product Name

Recombinant Rat DNA mismatch repair protein Mlh1 (Mlh1)

Product Gene Name

Mlh1 recombinant protein

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sequence Positions
1-757aa, Full length protein
Sequence
MSFVAGVIRR LDETVVNRIA AGEVIQRPAN AIKEMTENCL DAKSTNIQVI VREGGLKLIQ IQDNGTGIRK EDLDIVCERF TTSKLQTFED LAMISTYGFR GEALASISHV AHVTITTKTA DGKCAYRASY SDGKLQAPPK PCAGNQGTLI TVEDLFYNII TRKKALKNPS EEYGKILEVV GRYSIHNSGI SFSVKKQGET VSDVRTLPNA TTVDNIRSIF GNAVSRELIE VGCEDKTLAF KMNGYISNAN YSVKKCIFLL FINHRLVESA ALKKAIEAVY AAYLPKNTHP FLYLILEISP QNVDVNVHPT KHEVHFLHEE SILERVQQHI ESKLLGSNSS RMYFTQTLLP GLAGPSGEAV KSTTGIASSS TSGSGDKVHA YQMVRTDSRD QKLDAFMQPV SRRLPSQPQD PVPGNRTEGS PEKAMQKDQE ISELPAPMEA AADSASLERE SVIGASEVVA PQRHPSSPGS SRKRHPEDSD VEMMENDSRK EMTAACYPRR RIINLTSVLS LQEEINDRGH ETLREMLRNH TFVGCVNPQW ALAQHQTKLY LLNTTKLSEE LFYQILIYDF ANFGVLRLPE PAPLFDFAML ALDSPESGWT EEDGPKEGLA EYIVEFLKKK AKMLADYFSV EIDEEGNLIG LPLLIDSYVP PLEGLPIFIL RLATEVNWDE EECFESLSKE CAVFYSIRKQ YILEESALSG QQSDMPGSPS KPWKWTVEHI IYKAFRSHLL PPKHFTEDGN VLQLANLPDL CKVFERC
3D Structure
ModBase 3D Structure for P97679
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Rat
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Mlh1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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NCBI/Uniprot data below describe general gene information for Mlh1. It may not necessarily be applicable to this product.
NCBI GI #
13591989
NCBI GeneID
81685
NCBI Accession #
NP_112315.1 [Other Products]
NCBI GenBank Nucleotide #
NM_031053.1 [Other Products]
UniProt Primary Accession #
P97679 [Other Products]
UniProt Related Accession #
P97679[Other Products]
Molecular Weight
84,540 Da
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NCBI Official Full Name
DNA mismatch repair protein Mlh1
NCBI Official Synonym Full Names
mutL homolog 1
NCBI Official Symbol
Mlh1??[Similar Products]
NCBI Protein Information
DNA mismatch repair protein Mlh1
UniProt Protein Name
DNA mismatch repair protein Mlh1
UniProt Synonym Protein Names
MutL protein homolog 1
Protein Family
DNA mismatch repair protein
UniProt Gene Name
Mlh1??[Similar Products]
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NCBI Summary for Mlh1
may be involved in DNA mismatch repair [RGD, Feb 2006]
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UniProt Comments for Mlh1
Heterodimerizes with Pms2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (Msh2-Msh6) or MutS beta (Msh2-Msh6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of Pms2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (Mlh1-Pms2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with Mlh3 to form MutL gamma which plays a role in meiosis ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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