Product Name
LMAN1, siRNA
Full Product Name
LMAN1 siRNA (Rat)
Product Synonym Names
ERGIC53; Protein ERGIC-53; ER-Golgi intermediate compartment 53 kDa protein; Lectin mannose-binding 1; p58
Product Gene Name
LMAN1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q62902
Specificity
LMAN1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat LMAN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of LMAN1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LMAN1 sirna
siRNA to inhibit LMAN1 expression using RNA interference
Applications Tested/Suitable for LMAN1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for LMAN1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_446338.1
[Other Products]
NCBI GenBank Nucleotide #
NM_053886.2
[Other Products]
UniProt Primary Accession #
Q62902
[Other Products]
UniProt Related Accession #
Q62902[Other Products]
Molecular Weight
57,957 Da
NCBI Official Full Name
protein ERGIC-53
NCBI Official Synonym Full Names
lectin, mannose-binding, 1
NCBI Official Symbol
Lman1??[Similar Products]
NCBI Official Synonym Symbols
p58
??[Similar Products]
NCBI Protein Information
protein ERGIC-53
UniProt Protein Name
Protein ERGIC-53
UniProt Synonym Protein Names
ER-Golgi intermediate compartment 53 kDa protein; Lectin mannose-binding 1; p58
UniProt Gene Name
Lman1??[Similar Products]
UniProt Synonym Gene Names
Ergic53??[Similar Products]
UniProt Entry Name
LMAN1_RAT
NCBI Summary for LMAN1
calcium-dependent lectin; involved in glycoprotein export from the ER [RGD, Feb 2006]
UniProt Comments for LMAN1
LMAN1: Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. Defects in LMAN1 are THE cause of factor V and factor VIII combined deficiency type 1 (F5F8D1); also known as multiple coagulation factor deficiency I (MCFD1). F5F8D1 is an autosomal recessive blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal.
Protein type: Membrane protein, integral
Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; sarcomere; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; ER-Golgi intermediate compartment; ER to Golgi transport vesicle
Molecular Function: mannose binding; identical protein binding; calcium ion binding
Biological Process: endoplasmic reticulum organization and biogenesis; ER to Golgi vesicle-mediated transport; protein transport; protein exit from endoplasmic reticulum; Golgi organization and biogenesis
Research Articles on LMAN1
1. the crystal structure of the carbohydrate recognition domain (CRD) of p58, the rat homologue of human ERGIC-53, to 1.46 A resolution
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
