Product Name
FRAG1 (Atad5), Polyclonal Antibody
Full Product Name
Goat anti-FRAG1 (mouse) Antibody
Product Synonym Names
Atad5; ATPase family, AAA domain containing 5; C17orf41; chromosome 17 open reading frame 41; FLJ12735; FRAG1; chromosome fragility associated gene 1; hypothetical protein LOC237877; RP23-328O2.1; C130052G03Rik; Atad5 antibody; ATPase family; AAA domain containing 5 antibody; C17orf41 antibody; chromosome 17 open reading frame 41 antibody; FLJ12735 antibody; FRAG1 antibody; chromosome fragility associated gene 1 antibody; hypothetical protein LOC237877 antibody; RP23-328O2.1 antibody; C130052G03Rik antibody; FRAG1 (mouse)
Product Gene Name
anti-Atad5 antibody
[Similar Products]
Product Synonym Gene Name
FRAG1[Similar Products]
Antibody/Peptide Pairs
FRAG1 peptide (MBS426856) is used for blocking the activity of FRAG1 antibody (MBS420637)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
QSKDQPLRKS QK
Species Reactivity
Expected from sequence similarity: Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QSKDQPLRKSQK, from the internal region of the protein sequence according to NP_001025027.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-Atad5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Atad5 antibody
Peptide ELISA (EIA)
Application Notes for anti-Atad5 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Preliminary experiments gave an approx 60kDa band in lysates of cell lines NIH3T3 and NSO after 0. 01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 204kDa according to NP_001025027.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
NCBI/Uniprot data below describe general gene information for Atad5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001025027.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001029856.2
[Other Products]
UniProt Secondary Accession #
Q3TMG5; Q3UW85; Q3V306; Q3V3T9; Q5SSK4; Q8BUH4; Q8CGG7[Other Products]
UniProt Related Accession #
Q4QY64[Other Products]
Molecular Weight
90,207 Da
NCBI Official Full Name
ATPase family AAA domain-containing protein 5
NCBI Official Synonym Full Names
ATPase family, AAA domain containing 5
NCBI Official Symbol
Atad5??[Similar Products]
NCBI Official Synonym Symbols
Gm17; FRAG1; C130052G03Rik
??[Similar Products]
NCBI Protein Information
ATPase family AAA domain-containing protein 5
UniProt Protein Name
ATPase family AAA domain-containing protein 5
UniProt Synonym Protein Names
Chromosome fragility-associated gene 1 protein
Protein Family
ATPase family AAA domain-containing protein
UniProt Gene Name
Atad5??[Similar Products]
UniProt Synonym Gene Names
Frag1??[Similar Products]
UniProt Entry Name
ATAD5_MOUSE
UniProt Comments for Atad5
ATAD5: Involved in DNA damage response. Involved in a RAD9A- related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis. Modulates the RAD9A interaction with BCL2 and thereby induces DNA damages- induced apoptosis. Belongs to the AAA ATPase family. 2 isoforms of the human protein are produced by alternative splicing.
Cellular Component: nucleus
Molecular Function: ATP binding; nucleotide binding
Biological Process: response to DNA damage stimulus
Research Articles on Atad5
1. Atad5+/m cells accumulate in the S phase, have decreased proliferation, and have reduced double-strand breaks, class switch recombination, and translocations
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
