Product Name
IRAK-4 (IRAK4), Polyclonal Antibody
Full Product Name
IRAK-4 Polyclonal Antibody
Product Synonym Names
IRAK-4
Product Gene Name
anti-IRAK4 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 12; NC_000012.11 (44152747..44183346). Location: 12q12
3D Structure
ModBase 3D Structure for Q69FE3
Specificity
IRAK-4 [Rabbit polyclonal antibody to IRAK-4]
This antibody specifically recognizes human IRAK-4 (IL-1 Receptor Associated Kinase). IRAK-4 activates the NF- kappa B and MAPK pathways, and plays a role in IL-1R mediated inflammatory responses and innate immunity. Anti-IRAK-4 does not crossreact with other IRAKs.
Form/Format
100 ug IgG in PBS, pH 7.4,purified by immunoaffinity chromatography.
Antigen
A synthetic peptide corresponding to the carboxy terminus of human IRAK-4.
Preservatives
0.02% sodium azide
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
Store at 4 degree C; stable for one year
Other Notes
Small volumes of anti-IRAK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-IRAK4 antibody
Neuroscience and Signal Transduction Antibodies
Applications Tested/Suitable for anti-IRAK4 antibody
Western Blot
Application Notes for anti-IRAK4 antibody
Western Blot: Use at 1-2 ug/ml. A band of approximately 50 kD is detected using HeLa Cell Lysate as a positive control.
NCBI/Uniprot data below describe general gene information for IRAK4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001107654.1
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NCBI GenBank Nucleotide #
NM_001114182.2
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UniProt Primary Accession #
Q69FE3
[Other Products]
UniProt Related Accession #
Q69FE3; Q7Z6A7; Q7Z6A8; Q9NWZ3[Other Products]
Molecular Weight
51,530 Da[Similar Products]
NCBI Official Full Name
interleukin-1 receptor-associated kinase 4 isoform a
NCBI Official Synonym Full Names
interleukin-1 receptor-associated kinase 4
NCBI Official Symbol
IRAK4??[Similar Products]
NCBI Official Synonym Symbols
IPD1; REN64; NY-REN-64
??[Similar Products]
NCBI Protein Information
interleukin-1 receptor-associated kinase 4; IRAK-4; renal carcinoma antigen NY-REN-64
UniProt Protein Name
Interleukin-1 receptor associated kinase-4
Protein Family
Interleukin-1 receptor-associated kinase
UniProt Gene Name
IRAK4??[Similar Products]
UniProt Entry Name
Q69FE3_HUMAN
NCBI Summary for IRAK4
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq]
UniProt Comments for IRAK4
IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.
Protein type: Kinase, protein; Protein kinase, TKL; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; IRAK family
Chromosomal Location of Human Ortholog: 12q12
Cellular Component: extracellular space; cytoplasm; plasma membrane; endosome membrane; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; magnesium ion binding; ATP binding; protein kinase activity
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of smooth muscle cell proliferation; cytokine and chemokine mediated signaling pathway; cytokine production; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway
Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency
Research Articles on IRAK4
1. These results indicate that IL-1beta-induced IL-6 production in A549 cells is mediated by both PI3K and IRAK4 and suggest that involvement of PI3K in the IL-1-induced IL-6 production is cell type specific.
Precautions
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Disclaimer
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