MTAP; MSAP; c86fus

For Research Use Only. Not for use in diagnostic procedures.

IgG

Rabbit

Affinity purification

Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Small volumes of anti-MTAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MTAP is an enzyme that is essential for the salvage pathway for both adenine and methionine synthesis. MTAP catalyzes the cleavage of 5'-methylthioadenosine into adenine and 5-methylthio-D-ribose-1-phosphate. Adenine is then used to generate AMP whereas 5-methylthio-D-ribose-1-phosphate is converted into methionine (1, 2). MTAP is expressed in all normal cells and tissues, although frequently lost in different human tumors including pancreatic adenocarcinoma, neuroendocrine tumors, non-small cell lung carcinoma and breast carcinoma. MTAP is usually codeleted with p16 (cdkN2a/ARF) (3-5). MTAP overexpression in breast cancer cells inhibits their ability to form colonies in soft agar, thereby implicating its function as a tumor suppressor (6).

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

Western Blot (WB), Immunohistochemistry (IHC)

WB: 1:500-1:2000
IHC: 1:50-1:200
Western blot analysis of HT-29 cell and liver cell lysate using MTAP antibody.

31kDa

methylthioadenosine phosphorylase

S-methyl-5'-thioadenosine phosphorylase

S-methyl-5'-thioadenosine phosphorylase

MTA phosphorylase; MTAP; MTAPase??[Similar Products]

This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.

1. Backlund, P.S. and Smith, R.A. (1981) J Biol Chem 256, 1533-5. 2. Backlund, P.S. et al. (1982) J Biol Chem 257, 4196-202. 3. Dreyling, M.H. et al. (1998) Genes Chromosomes Cancer 22, 72-8. 4. Zhang, H. et al. (1996) Cancer Genet Cytogenet 86, 22-8. 5. Illei, P.B. et al. (2003) Clin Cancer Res 9, 2108-13. 6. Christopher, S.A. et al. (2002) Cancer Res 62, 6639-44.

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