Full Product Name
MTAP siRNA (Human)
Product Synonym Names
MSAP; S-methyl-5'-thioadenosine phosphorylase; 5'-methylthioadenosine phosphorylase; MTA phosphorylase; MTAP; MTAPase
Product Gene Name
MTAP sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13126
Specificity
MTAP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MTAP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MTAP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MTAP sirna
siRNA to inhibit MTAP expression using RNA interference
Applications Tested/Suitable for MTAP sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MTAP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002442.2
[Other Products]
NCBI GenBank Nucleotide #
NM_002451.3
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UniProt Primary Accession #
Q13126
[Other Products]
UniProt Secondary Accession #
Q5T3P3; Q9H010; I2G7M5; I2G7M6; I2G7M7; I2G7M8; I2G7M9; I2G7N0[Other Products]
UniProt Related Accession #
Q13126[Other Products]
Molecular Weight
26,278 Da
NCBI Official Full Name
S-methyl-5'-thioadenosine phosphorylase
NCBI Official Synonym Full Names
methylthioadenosine phosphorylase
NCBI Official Symbol
MTAP??[Similar Products]
NCBI Official Synonym Symbols
BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249
??[Similar Products]
NCBI Protein Information
S-methyl-5'-thioadenosine phosphorylase
UniProt Protein Name
S-methyl-5'-thioadenosine phosphorylase
UniProt Synonym Protein Names
5'-methylthioadenosine phosphorylase
UniProt Gene Name
MTAP??[Similar Products]
UniProt Synonym Gene Names
MTA phosphorylase; MTAP; MTAPase??[Similar Products]
UniProt Entry Name
MTAP_HUMAN
NCBI Summary for MTAP
This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]
UniProt Comments for MTAP
MTAP: Catalyzes the reversible phosphorylation of S-methyl-5'- thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S- adenosylmethionine. Has broad substrate specificity with 6- aminopurine nucleosides as preferred substrates. Homotrimer. Ubiquitously expressed. Inhibited by 5'-methylthiotubercin and 5'- chloroformycin. Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.
Protein type: EC 2.4.2.28; Amino Acid Metabolism - cysteine and methionine; Transferase; Phosphorylase
Chromosomal Location of Human Ortholog: 9p21
Cellular Component: cytoplasm; nucleus; cytosol
Molecular Function: phosphorylase activity; S-methyl-5-thioadenosine phosphorylase activity
Biological Process: methylation; methionine salvage; nicotinamide riboside catabolic process; sulfur amino acid metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; response to testosterone stimulus; purine ribonucleoside salvage; polyamine metabolic process
Research Articles on MTAP
1. MTAP expression is an independent prognostic factor and has greater prognostic significance than p16 expression in non-small cell lung cancer and concordant loss of MTAP and p16 expression indicates poor outcomes in lung cancer patients.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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