Product Name
PITX1, Polyclonal Antibody
Popular Item
Full Product Name
PITX1 Polyclonal Antibody
Product Synonym Names
BFT; CCF; POTX; PTX1; LBNBG
Product Gene Name
anti-PITX1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P78337
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human PITX1
Calculated Molecular Weight
34kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-PITX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PITX1 antibody
This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin.
Product Categories/Family for anti-PITX1 antibody
Polyclonal
Applications Tested/Suitable for anti-PITX1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-PITX1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:100
Western Blot (WB) of anti-PITX1 antibody
Western blot analysis of extracts of rat pancreas, using PITX1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
NCBI/Uniprot data below describe general gene information for PITX1. It may not necessarily be applicable to this product.
NCBI Accession #
P78337.2
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UniProt Primary Accession #
P78337
[Other Products]
UniProt Secondary Accession #
O14677; O60425; Q9BTI5; A8K3M0; D3DQB0[Other Products]
UniProt Related Accession #
P78337[Other Products]
NCBI Official Full Name
Pituitary homeobox 1
NCBI Official Synonym Full Names
paired-like homeodomain 1
NCBI Official Symbol
PITX1??[Similar Products]
NCBI Official Synonym Symbols
BFT; CCF; POTX; PTX1; LBNBG
??[Similar Products]
NCBI Protein Information
pituitary homeobox 1; pituitary homeo box 1; homeobox protein PITX1; pituitary otx-related factor; hindlimb expressed homeobox protein backfoot; hindlimb-expressed homeobox protein backfoot; paired-like homeodomain transcription factor 1
UniProt Protein Name
Pituitary homeobox 1
UniProt Synonym Protein Names
Hindlimb-expressed homeobox protein backfoot; Homeobox protein PITX1; Paired-like homeodomain transcription factor 1
Protein Family
Pituitary homeobox
UniProt Gene Name
PITX1??[Similar Products]
UniProt Synonym Gene Names
BFT; PTX1??[Similar Products]
UniProt Entry Name
PITX1_HUMAN
NCBI Summary for PITX1
This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family are involved in organ development and left-right asymmetry. This protein acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. [provided by RefSeq, Jul 2008]
UniProt Comments for PITX1
PITX1: May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. Defects in PITX1 are a cause of congenital clubfoot (CCF); also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait. Belongs to the paired homeobox family. Bicoid subfamily.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: transcription factor complex; cytoplasm; nucleolus; nucleus
Biological Process: myoblast cell fate commitment; anatomical structure morphogenesis; transcription, DNA-dependent; pituitary gland development; cartilage development; branchiomeric skeletal muscle development; positive regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; skeletal development
Disease: Clubfoot, Congenital, With Or Without Deficiency Of Long Bones And/or Mirror-image Polydactyly; Liebenberg Syndrome
Research Articles on PITX1
1. DUX4 gene is activated in a small number of myonuclei, the DUX4 proteins diffuse to adjacent nuclei where they activate target genes such as PITX1.
Precautions
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Disclaimer
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