Product Name
Interleukin 1 Receptor Associated Kinase 4 (IRAK4), Antibody Pair Kit
Full Product Name
Interleukin 1 Receptor Associated Kinase 4 (IRAK4) Antibody Pair Kit (with Standard)
Product Synonym Names
NY-REN-64
Product Gene Name
IRAK4 antibody pair kit
[Similar Products]
Matching Pairs
Antibody: Interleukin 1 Receptor Associated Kinase 4 (IRAK4) (MBS2100507)
Pairing: Interleukin 1 Receptor Associated Kinase 4 (IRAK4) (MBS2100507)
Comments: Matched antibody pair kit containing antibody/standard combination
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
Samples
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
3.12-200ng/mL
Standard
Recombinant IRAK4 (Lyophilized, 1 vial-10ug)
Capture Antibody
Rabbit polyclonal antibody (Liquid, 1 vial-400mug/800muL)
Detection Antibody
Biotinylated Rabbit polyclonal antibody (Liquid, 1 vial-100mug/500muL)
Producte Usage
Raw materials can be used for preparation of 5x96T or 10x96T ELISA Kits.
Antibody Support Pack
Antibody Support Pack (Buffer Pack) is sold separately as catalog #MBS2090685
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IRAK4 antibody pair kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for IRAK4 antibody pair kit
Signal transduction; Enzyme & Kinase; Apoptosis; Tumor immunity; Infection immunity; Pulmonology
Applications Tested/Suitable for IRAK4 antibody pair kit
ELISA (EIA), Chemiluminescence Immunoassays (CLIA), ELISPOT, Luminex
NCBI/Uniprot data below describe general gene information for IRAK4. It may not necessarily be applicable to this product.
NCBI Accession #
AAM15772.1
[Other Products]
UniProt Secondary Accession #
Q69FE1; Q8TDF7; Q9Y589[Other Products]
UniProt Related Accession #
Q9NWZ3[Other Products]
Molecular Weight
37,674 Da
NCBI Official Full Name
interleukin-1 receptor associated kinase 4
NCBI Official Synonym Full Names
interleukin 1 receptor associated kinase 4
NCBI Official Symbol
IRAK4??[Similar Products]
NCBI Official Synonym Symbols
IPD1; REN64; IRAK-4; NY-REN-64
??[Similar Products]
NCBI Protein Information
interleukin-1 receptor-associated kinase 4
UniProt Protein Name
Interleukin-1 receptor-associated kinase 4
UniProt Synonym Protein Names
Renal carcinoma antigen NY-REN-64
Protein Family
Interleukin-1 receptor-associated kinase
UniProt Gene Name
IRAK4??[Similar Products]
UniProt Synonym Gene Names
IRAK-4??[Similar Products]
NCBI Summary for IRAK4
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniProt Comments for IRAK4
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
Research Articles on IRAK4
1. Data suggest that IRAK4 activity regulates activation of IRF5, TAK1, and IKKB in monocytes; IRAK4 activation of TAK1-IKKB-IRF5 axis leads to induction of cytokines and interferons following TLR7/TLR8 stimulation. (IRAK4 = interleukin-1 receptor-associated kinase 4; IRF5 = interferon regulatory factor-5; TAK1 = MAPK kinase kinase 7; IKKB = I-kappa B kinase; TLR = toll-like receptor)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.