Full Product Name
Anti-VDR Antibody
Product Synonym Names
Vitamin D3 receptor; 1 25 dihydroxyvitamin D3 receptor; 1; 1,25-@dihydroxyvitamin D3 receptor; 125 dihydroxyvitamin D3 receptor; 25-dihydroxyvitamin D3 receptor; Member 1; NR1I1; Nuclear receptor subfamily 1 group I member 1; VDR; VDR_HUMAN; Vitamin D (1,25- dihydroxyvitamin D3) receptor; Vitamin D hormone receptor; Vitamin D nuclear receptor variant 1; Vitamin D receptor; Vitamin D3 receptor antibody; vitamin D (1,25- dihydroxyvitamin D3) receptor
Product Gene Name
anti-VDR antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P11473
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human VDR (377-402aa HLLYAKMIQKLADLRSLNEEHSKQYR), different from the related mouse and rat sequences by one amino acid.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-VDR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-VDR antibody
Description: Rabbit IgG polyclonal antibody for Vitamin D3 receptor(VDR) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Background: VDR (Vitamin D Receptor), also known as Vitamin D Hormone Receptor, is a member of the nuclear receptor family of transcription factors. Labuda et al. (1991) assigned the VDR gene to 12q12-q14 by in situ hybridization. Using mutation analysis, Jurutka et al. (2000) characterized arg18/arg22, VDR residues immediately N-terminal of the first DNA-binding zinc finger, as vital for contact with the general transcription factor IIB (TFIIB). A natural polymorphic variant of VDR, termed F/M4 (missing a FokI restriction site), which lacks only the first 3 amino acids (including glu2), interacted more efficiently with TFIIB and also possessed elevated transcriptional activity compared with the full-length (f/M1) receptor. Shah et al. (2006) stated that the signaling and oncogenic activity of beta-catenin (CTNNB1) can be repressed by activation of VDR. Conversely, high levels of beta-catenin can potentiate the transcriptional activity of 1,25- dihydroxyvitamin D3.
Applications Tested/Suitable for anti-VDR antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-VDR antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
Western Blot (WB) of anti-VDR antibody
Anti-VDR Picoband antibody, MBS178395, Western blotting
All lanes: Anti VDR (MBS178395) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Pancreas Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD

Immunohistochemistry (IHC) of anti-VDR antibody
Anti-VDR Picoband antibody, MBS178395, IHC(P)
IHC(P): Mouse Intestine Tissue

Immunohistochemistry (IHC) of anti-VDR antibody
Anti-VDR Picoband antibody, MBS178395, IHC(P)
IHC(P): Rat Intestine Tissue

Immunohistochemistry (IHC) of anti-VDR antibody
Anti-VDR Picoband antibody, MBS178395, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

NCBI/Uniprot data below describe general gene information for VDR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000367.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000376.2
[Other Products]
UniProt Primary Accession #
P11473
[Other Products]
UniProt Secondary Accession #
Q5PSV3; B2R5Q1; G3V1V9[Other Products]
UniProt Related Accession #
P11473[Other Products]
Molecular Weight
53,883 Da
NCBI Official Full Name
vitamin D3 receptor isoform VDRA
NCBI Official Synonym Full Names
vitamin D (1,25- dihydroxyvitamin D3) receptor
NCBI Official Symbol
VDR??[Similar Products]
NCBI Official Synonym Symbols
NR1I1; PPP1R163
??[Similar Products]
NCBI Protein Information
vitamin D3 receptor
UniProt Protein Name
Vitamin D3 receptor
UniProt Synonym Protein Names
1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1
Protein Family
Vitamin D3 receptor
UniProt Gene Name
VDR??[Similar Products]
UniProt Synonym Gene Names
NR1I1; VDR??[Similar Products]
UniProt Entry Name
VDR_HUMAN
NCBI Summary for VDR
This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Feb 2011]
UniProt Comments for VDR
Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
Product References and Citations for anti-VDR antibody
1. Jurutka, P. W., Remus, L. S., Whitfield, G. K., Thompson, P. D., Hsieh, J.-C., Zitzer, H., Tavakkoli, P., Galligan, M. A., Dang, H. T. L., Haussler, C. A., Haussler, M. R. The polymorphic N terminus in human vitamin D receptor isoforms influences transcriptional activity by modulating interaction with transcription factor IIB. Molec. Endocr. 14: 401-420, 2000. 2. Labuda, M., Ross, M. V., Fujiwara, T. M., Morgan, K., Ledbetter, D., Hughes, M. R., Glorieux, F. H. Two hereditary defects related to vitamin D metabolism map to the same region of human chromosome 12q.II(Abstract) Cytogenet. Cell Genet. 58: 1978, 1991. 3. Shah, S., Islam, M. N., Dakshanamurthy, S., Rizvi, I., Rao, M., Herrell, R., Zinser, G., Valrance, M., Aranda, A., Moras, D., Norman, A., Welsh, J., Byers, S. W. The molecular basis of vitamin D receptor and beta-catenin crossregulation. Molec. Cell 21: 799-809, 2006. Note: Erratum: Molec. Cell 21: 148 only, 2006.
Research Articles on VDR
1. The VDR and CYP2R1 variants may be involved in genetic interactions in the pathogenesis of persistent allergic rhinitis.
Precautions
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Disclaimer
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