Product Name
Cystatin B (CSTB), Monoclonal Antibody
Full Product Name
Cystatin B Antibody with Control Lysate
Product Synonym Names
Mouse anti CYSTATIN B; Cystatin B Antibody
Product Gene Name
anti-CSTB antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P04080
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Immunogen
Recombinant human cystatin B
Lysate Composition
400ug A549 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Preparation and Storage
Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Other Notes
Small volumes of anti-CSTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CSTB antibody
Western Blot (WB)
Application Notes for anti-CSTB antibody
WB: 1:1000
Western Blotting: Detects a band of approximately 37 kDa in A549 cell lysates.
Western Blot (WB) of anti-CSTB antibody
Western blot analysis of whole cell lysates probed with cystatin B antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 3.34 second exposure. Arrow points to cystatin B (molecular weight 37 kDa)
NCBI/Uniprot data below describe general gene information for CSTB. It may not necessarily be applicable to this product.
NCBI Accession #
P04080.2
[Other Products]
UniProt Primary Accession #
P04080
[Other Products]
UniProt Related Accession #
P04080[Other Products]
NCBI Official Full Name
Cystatin-B
NCBI Official Synonym Full Names
cystatin B
NCBI Official Symbol
CSTB??[Similar Products]
NCBI Official Synonym Symbols
PME; ULD; CST6; EPM1; STFB; CPI-B; EPM1A
??[Similar Products]
NCBI Protein Information
cystatin-B
UniProt Protein Name
Cystatin-B
UniProt Synonym Protein Names
CPI-B; Liver thiol proteinase inhibitor; Stefin-B
UniProt Gene Name
CSTB??[Similar Products]
UniProt Synonym Gene Names
CST6; STFB??[Similar Products]
NCBI Summary for CSTB
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies. [provided by RefSeq, Jul 2016]
UniProt Comments for CSTB
This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
Research Articles on CSTB
1. The results demonstrate that cystatin B interferes with the STAT-1 signaling and IFN-beta-antiviral responses perpetuating HIV in macrophage reservoirs.
Precautions
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Disclaimer
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