Product Name
CHAF1A, siRNA
Full Product Name
CHAF1A siRNA (Mouse)
Product Synonym Names
CAIP150; Chromatin assembly factor 1 subunit A; CAF-1 subunit A; Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150
Product Gene Name
CHAF1A sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9QWF0
Specificity
CHAF1A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHAF1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CHAF1A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CHAF1A sirna
siRNA to inhibit CHAF1A expression using RNA interference
Applications Tested/Suitable for CHAF1A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CHAF1A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_038761.1
[Other Products]
NCBI GenBank Nucleotide #
NM_013733.3
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UniProt Primary Accession #
Q9QWF0
[Other Products]
UniProt Secondary Accession #
Q3TU22; Q544M2[Other Products]
UniProt Related Accession #
Q9QWF0[Other Products]
Molecular Weight
101,936 Da
NCBI Official Full Name
chromatin assembly factor 1 subunit A
NCBI Official Synonym Full Names
chromatin assembly factor 1, subunit A (p150)
NCBI Official Symbol
Chaf1a??[Similar Products]
NCBI Official Synonym Symbols
CAF-1; Cac1p; AL023013; AL024058
??[Similar Products]
NCBI Protein Information
chromatin assembly factor 1 subunit A
UniProt Protein Name
Chromatin assembly factor 1 subunit A
UniProt Synonym Protein Names
Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150
Protein Family
Chromatin assembly factor
UniProt Gene Name
Chaf1a??[Similar Products]
UniProt Synonym Gene Names
Caip150; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150??[Similar Products]
UniProt Entry Name
CAF1A_MOUSE
UniProt Comments for CHAF1A
CAF-1A: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci. The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase- specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with CBX5. Belongs to the CHAF1A family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA replication
Cellular Component: protein complex; nuclear chromatin; nucleus
Molecular Function: identical protein binding; protein binding
Biological Process: nucleosome assembly; transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin assembly; DNA replication; cell cycle; DNA repair; DNA replication-dependent nucleosome assembly; response to DNA damage stimulus
Research Articles on CHAF1A
1. Findings suggest that p150 may link the DNA-bound Gfi1 repressor complex to histones enabling modifications required for transcriptional silencing.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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