CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous level of total CHAF1B antibody.

Antibodies were purified by affinity purification using immunogen.

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-CHAF1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.

Total protein Ab

Western Blot (WB), Immunofluorescence (IF)

Western blotting: 1:500 - 1:2000
Immunofluorescence: 1:20 - 1:100

Western blot analysis of extracts of various cell lines, using CHAF1B antibody.

Immunofluorescence analysis of MCF-7 cell using CHAF1B antibody. Blue: DAPI for nuclear staining.

61,493 Da

chromatin assembly factor 1, subunit B (p60)

chromatin assembly factor 1 subunit B

Chromatin assembly factor 1 subunit B

CAF1A; CAF1P60; MPHOSPH7; MPP7; CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60??[Similar Products]

CAF1B_HUMAN

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]

CAF-1B: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. Belongs to the WD repeat HIR1 family.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 21q22.13

Cellular Component: nucleoplasm; protein complex; nuclear chromatin; cytoplasm; nucleus; cytosol

Molecular Function: histone binding; unfolded protein binding; chromatin binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin assembly; protein complex assembly; cell cycle; reproduction; DNA repair; DNA replication; DNA replication-dependent nucleosome assembly

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