Product Name
CHAF1B, siRNA
Full Product Name
CHAF1B siRNA (Human)
Product Synonym Names
CAF1A; CAF1P60; MPHOSPH7; MPP7; Chromatin assembly factor 1 subunit B; CAF-1 subunit B; Chromatin assembly factor I p60 subunit; CAF-I 60 kDa subunit; CAF-I p60; M-phase phosphoprotein 7
Product Gene Name
CHAF1B sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13112
Specificity
CHAF1B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CHAF1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CHAF1B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CHAF1B sirna
siRNA to inhibit CHAF1B expression using RNA interference
Applications Tested/Suitable for CHAF1B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CHAF1B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005432.1
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NCBI GenBank Nucleotide #
NM_005441.2
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UniProt Primary Accession #
Q13112
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UniProt Secondary Accession #
Q99548[Other Products]
UniProt Related Accession #
Q13112[Other Products]
Molecular Weight
61,493 Da
NCBI Official Full Name
chromatin assembly factor 1 subunit B
NCBI Official Synonym Full Names
chromatin assembly factor 1, subunit B (p60)
NCBI Official Symbol
CHAF1B??[Similar Products]
NCBI Official Synonym Symbols
CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7
??[Similar Products]
NCBI Protein Information
chromatin assembly factor 1 subunit B
UniProt Protein Name
Chromatin assembly factor 1 subunit B
UniProt Synonym Protein Names
Chromatin assembly factor I p60 subunit; CAF-I 60 kDa subunit; CAF-I p60; M-phase phosphoprotein 7
Protein Family
Chromatin assembly factor
UniProt Gene Name
CHAF1B??[Similar Products]
UniProt Synonym Gene Names
CAF1A; CAF1P60; MPHOSPH7; MPP7; CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60??[Similar Products]
UniProt Entry Name
CAF1B_HUMAN
NCBI Summary for CHAF1B
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]
UniProt Comments for CHAF1B
CAF-1B: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. Belongs to the WD repeat HIR1 family.
Protein type: DNA replication
Chromosomal Location of Human Ortholog: 21q22.13
Cellular Component: nucleoplasm; protein complex; cytoplasm; nuclear chromatin; nucleus; cytosol
Molecular Function: histone binding; unfolded protein binding; chromatin binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin assembly; protein complex assembly; cell cycle; DNA repair; reproduction; DNA replication; DNA replication-dependent nucleosome assembly
Research Articles on CHAF1B
1. The levels of the EDNRB, HJURP and p60/CAF-1 proteins were strongly associated with overall survival in high-grade gliomas patients (p<0.001, p<0.001 and p=0.002, respectively), whereas the one of PDLI4 was not (P=0.11).
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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