For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Affinity Purified

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CHAF1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This is a rabbit polyclonal antibody against CHAF1B. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. CHAF1B corresponds to the p60 subunit and is required for chromatin assembly after replication. CHAF1B is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Polyclonal; Cell Cycle Proteins; Transcription Regulation; Down Syndrome; Chromatin & Nuclear Signaling; Epigenetics; DNA Damage & Repair; Meiosis/Mitosis/Cell Cycle; DNA Repair; Transcription Factors;

Western Blot (WB)

Sample Type: Mouse brains
CHAF1B antibody - N-terminal region validated by WB using Mouse brains at 1:1000.

Host: Rabbit
Target Name: CHAF1B
Sample Type: 293T
Antibody Dilution: 1.0ug/mlCHAF1B is supported by BioGPS gene expression data to be expressed in HEK293T

Host: Rabbit
Target Name: CHAF1B
Sample Type: HepG2
Antibody Dilution: 1.0ug/mlCHAF1B is supported by BioGPS gene expression data to be expressed in HepG2

Host: Rabbit
Target Name: CHAF1B
Sample Type: Human Adult Placenta
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: CHAF1B
Sample Type: Jurkat
Antibody Dilution: 1.0ug/mlCHAF1B is supported by BioGPS gene expression data to be expressed in Jurkat

WB Suggested Anti-CHAF1B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Liver

61kDa

chromatin assembly factor 1 subunit B

chromatin assembly factor 1 subunit B

Chromatin assembly factor 1 subunit B

CAF1A; CAF1P60; MPHOSPH7; MPP7; CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60??[Similar Products]

CAF1B_HUMAN

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]

CAF-1B: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. Belongs to the WD repeat HIR1 family.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 21q22.13

Cellular Component: nucleoplasm; protein complex; cytoplasm; nuclear chromatin; cytosol; nucleus

Molecular Function: histone binding; unfolded protein binding; chromatin binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin assembly; protein complex assembly; cell cycle; reproduction; DNA repair; DNA replication; DNA replication-dependent nucleosome assembly

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