Product Name
CSTB, Polyclonal Antibody
Popular Item
Full Product Name
CSTB Antibody
Product Synonym Names
CHROW21; CPI B; CPI-B; CST 6; CST6; CSTB; Cystatin B (stefin B); Cystatin B; Cystatin-B; CYTB; CYTB_HUMAN; EPM1; EPM1A; Liver thiol proteinase inhibitor; PME; Stefin-B; STF B; STFB; ULD
Product Gene Name
anti-CSTB antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P04080
Species Reactivity
Human, Rat
Specificity
CSTB antibody detects endogenous levels of total CSTB
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human CSTB
Subcellular Location
Cytoplasm. Nucleus.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog
Similarity
Pig (83%), Bovine (83%), Sheep (83%), Rabbit (83%), Dog (100%)
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-CSTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CSTB antibody
Description: CSTB This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Belongs to the cystatin family. Able to form dimers stabilized by noncovalent forces.
Function: This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
Subunit Structure: Able to form dimers stabilized by noncovalent forces.
Similarity: Belongs to the cystatin family.
Applications Tested/Suitable for anti-CSTB antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-CSTB antibody
WB: 1:500-1:3000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
Immunohistochemistry (IHC) of anti-CSTB antibody
MBS9600193 at 1/100 staining human lung carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Immunofluorescene (IF) of anti-CSTB antibody
MBS9600193 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

Western Blot (WB) of anti-CSTB antibody
Western blot analysis on A549 cell lysate using CSTB Antibody, The lane on the left is treated with the antigen-specific peptide.

Immunofluorescence (IF) of anti-CSTB antibody
MBS9600193 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

Immunofluorescence (IF) of anti-CSTB antibody
MBS9600193 staining A549? cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

NCBI/Uniprot data below describe general gene information for CSTB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000091.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000100.3
[Other Products]
UniProt Primary Accession #
P04080
[Other Products]
UniProt Related Accession #
P04080[Other Products]
Molecular Weight
Observed: 11 kDa
Predicted: 12 kDa
NCBI Official Full Name
cystatin-B
NCBI Official Synonym Full Names
cystatin B
NCBI Official Symbol
CSTB??[Similar Products]
NCBI Official Synonym Symbols
PME; ULD; CST6; EPM1; STFB; CPI-B; EPM1A
??[Similar Products]
NCBI Protein Information
cystatin-B
UniProt Protein Name
Cystatin-B
UniProt Synonym Protein Names
CPI-B; Liver thiol proteinase inhibitor; Stefin-B
UniProt Gene Name
CSTB??[Similar Products]
UniProt Synonym Gene Names
CST6; STFB??[Similar Products]
NCBI Summary for CSTB
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies. [provided by RefSeq, Jul 2016]
UniProt Comments for CSTB
This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
Research Articles on CSTB
1. cystatin B expression was significantly and inversely correlated with lung tumor stage and tumor grade
Precautions
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Disclaimer
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