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Calpain 10 (CAPN10), ELISA Kit

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產(chǎn)品名稱: Calpain 10 (CAPN10), ELISA Kit
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Calpain 10 (CAPN10), ELISA Kit


Calpain 10 (CAPN10), ELISA Kit  的詳細(xì)介紹
Product Name

Calpain 10 (CAPN10), ELISA Kit

Full Product Name

Human Calpain 10 (CAPN10) ELISA Kit

Product Gene Name

CAPN10 elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Request for Current Manual Insert
Request Current Manual
OMIM
605286
Species Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of CAPN10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CAPN10 purchase
MBS7221156 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calpain 10 (CAPN10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CAPN10. The ELISA analytical biochemical technique of the MBS7221156 kit is based on CAPN10 antibody-CAPN10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CAPN10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CAPN10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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NCBI/Uniprot data below describe general gene information for CAPN10. It may not necessarily be applicable to this product.
NCBI GI #
37589919
NCBI GeneID
11132
NCBI Accession #
AAH07553.2 [Other Products]
UniProt Secondary Accession #
Q4ZFV1; Q8NCD4; Q96IG4; Q96JI2; Q9HC89; Q9HC90; Q9HC91; Q9HC92; Q9HC93; Q9HC94; A8MVS7[Other Products]
UniProt Related Accession #
Q9HC96[Other Products]
Molecular Weight
15,275 Da
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NCBI Official Full Name
Calpain 10
NCBI Official Synonym Full Names
calpain 10
NCBI Official Symbol
CAPN10??[Similar Products]
NCBI Official Synonym Symbols
CANP10; NIDDM1
??[Similar Products]
NCBI Protein Information
calpain-10; calpain-like protease CAPN10; calcium-activated neutral proteinase 10
UniProt Protein Name
Calpain-10
UniProt Synonym Protein Names
Calcium-activated neutral proteinase 10; CANP 10
Protein Family
Calpain
UniProt Gene Name
CAPN10??[Similar Products]
UniProt Synonym Gene Names
KIAA1845; CANP 10??[Similar Products]
UniProt Entry Name
CAN10_HUMAN
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NCBI Summary for CAPN10
Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010]
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UniProt Comments for CAPN10
CAPN10: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Defects in CAPN10 are a cause of susceptibility to diabetes mellitus non-insulin-dependent type 1 (NIDDM1). It is a multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Belongs to the peptidase C2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Protease; EC 3.4.22.-; Apoptosis

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: mitochondrion; cell; plasma membrane; cytosol

Molecular Function: SNARE binding; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity

Biological Process: positive regulation of glucose import; cellular response to insulin stimulus; positive regulation of intracellular transport; actin cytoskeleton reorganization; positive regulation of insulin secretion; proteolysis; cell structure disassembly during apoptosis

Disease: Diabetes Mellitus, Noninsulin-dependent, 1
Research Articles on CAPN10
1. Variations of SNP-43, -63 and Indel-19 of CAPN10 were not associated with an increased risk of developing gestational diabetes mellitus.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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