Full Product Name
CSTB siRNA (Rat)
Product Synonym Names
CST6; Cystatin-B; Cystatin-beta; Liver thiol proteinase inhibitor; Stefin-B
Product Gene Name
CSTB sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01041
Specificity
CSTB siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CSTB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CSTB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CSTB sirna
siRNA to inhibit CSTB expression using RNA interference
Applications Tested/Suitable for CSTB sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CSTB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036970.1
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NCBI GenBank Nucleotide #
NM_012838.2
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UniProt Primary Accession #
P01041
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UniProt Related Accession #
P01041[Other Products]
Molecular Weight
11,196 Da
NCBI Official Full Name
cystatin-B
NCBI Official Synonym Full Names
cystatin B (stefin B)
NCBI Official Symbol
Cstb??[Similar Products]
NCBI Official Synonym Symbols
Cyb; Epm1; Stfb
??[Similar Products]
NCBI Protein Information
cystatin-B
UniProt Protein Name
Cystatin-B
UniProt Synonym Protein Names
Cystatin-beta; Liver thiol proteinase inhibitor; Stefin-B
UniProt Gene Name
Cstb??[Similar Products]
UniProt Synonym Gene Names
Cst6??[Similar Products]
UniProt Entry Name
CYTB_RAT
NCBI Summary for CSTB
inhibits the activity of cathepsins B, H, L, and S; may protect against apoptosis that occurs in response to seizures [RGD, Feb 2006]
UniProt Comments for CSTB
CSTB: This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Defects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1). EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop. Belongs to the cystatin family.
Protein type: Inhibitor; Apoptosis
Cellular Component: extracellular space; cytoplasm; nucleolus
Molecular Function: protein binding; protease binding; cysteine protease inhibitor activity
Biological Process: negative regulation of proteolysis; regulation of apoptosis; ***** locomotory behavior; negative regulation of peptidase activity
Research Articles on CSTB
1. A reciprocal influence of CSTB and SOD1 at the gene expression level and for a direct interaction of the two proteins, is reported.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
