Product Name
CSTB, Recombinant Protein
Full Product Name
Recombinant Mouse CSTB Protein
Product Synonym Names
CST6; STFB; Cystatin-B; CPI-B; Liver thiol proteinase inhibitor; Stefin-B
Product Gene Name
CSTB recombinant protein
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
ELRSRRTGER QNLSPTDPRV QKAAQAAVAS YNMGSDSLYY FRDTKVIDAK YQLVAGIKYY LTLDIESTEC RKTRVSGEHM DLTTCPLAAG GQQEKLRCNF ELLEVPWKNT TQLLKHDCVQ VVDHHHHHH
3D Structure
ModBase 3D Structure for Q62426
Purity/Purification
Greater than 95% as determined by reducing SDS-PAGE.
Form/Format
Supplied as a 0.2 muM filtered solution of 20mM MES, 150mM NaCl, pH 7.4.
Endotoxin
Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
Directions for Use
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Other Notes
Small volumes of CSTB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CSTB recombinant protein
Recombinant Mouse CSTB Protein is produced by our mammalian expression system and the target gene encoding Glu29-Val149 is expressed with a 6His tag at the C-terminus.
Applications Tested/Suitable for CSTB recombinant protein
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
NCBI/Uniprot data below describe general gene information for CSTB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_031819.1
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NCBI GenBank Nucleotide #
NM_007793.3
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UniProt Primary Accession #
Q62426
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UniProt Secondary Accession #
Q3UAW2[Other Products]
UniProt Related Accession #
Q62426[Other Products]
Molecular Weight
11,046 Da
NCBI Official Full Name
cystatin-B
NCBI Official Synonym Full Names
cystatin B
NCBI Official Symbol
Cstb??[Similar Products]
NCBI Official Synonym Symbols
Stfb; AA960480
??[Similar Products]
NCBI Protein Information
cystatin-B
UniProt Protein Name
Cystatin-B
UniProt Synonym Protein Names
Stefin-B
UniProt Gene Name
Cstb??[Similar Products]
UniProt Synonym Gene Names
Cst6; Stfb??[Similar Products]
UniProt Entry Name
CYTB_MOUSE
UniProt Comments for CSTB
CSTB: This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Defects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1). EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop. Belongs to the cystatin family.
Protein type: Inhibitor
Cellular Component: cytoplasm; extracellular space; nucleolus; nucleus
Molecular Function: cysteine protease inhibitor activity; endopeptidase inhibitor activity; protease binding
Biological Process: ***** locomotory behavior; negative regulation of peptidase activity; negative regulation of proteolysis
Research Articles on CSTB
1. Results suggest a role for CSTB in regulating microglial activation: data link CSTB deficiency to early activation and altered functional properties of microglia, imply presence of both enhanced and suppressed immune response-related microglial functions
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
