Product Name
CCKA Receptor (Cckar), Polyclonal Antibody
Full Product Name
Goat anti-CCKA Receptor (mouse) Antibody
Product Synonym Names
Cckar; cholecystokinin A receptor; AW106902; Cckar antibody; cholecystokinin A receptor antibody; AW106902 antibody; CCKA Receptor (mouse)
Product Gene Name
anti-Cckar antibody
[Similar Products]
Product Synonym Gene Name
CCKA[Similar Products]
Antibody/Peptide Pairs
CCKA Receptor peptide (MBS425052) is used for blocking the activity of CCKA Receptor antibody (MBS421309)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
KFDASQKKSA KEKR
Species Reactivity
Tested: Rat; Expected from sequence similarity: Mouse, Rat, Rabbit
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-KFDASQKKSAKEKR, from the internal region of the protein sequence according to NP_033957.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-Cckar antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Cckar antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-Cckar antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx.75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J. Int J Endocrinol.2009;2009: 875167; PMID: 20011057. The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem.2003 Nov;51(11): 1501-13; PMID: 14566022). Recommended concentration: 0.1-0.3ug/ml.
NCBI/Uniprot data below describe general gene information for Cckar. It may not necessarily be applicable to this product.
NCBI Accession #
NP_033957.1
[Other Products]
NCBI GenBank Nucleotide #
NM_009827.2
[Other Products]
UniProt Secondary Accession #
Q8VCC7; Q9DBV6[Other Products]
UniProt Related Accession #
O08786[Other Products]
Molecular Weight
48,437 Da
NCBI Official Full Name
cholecystokinin receptor type A
NCBI Official Synonym Full Names
cholecystokinin A receptor
NCBI Official Symbol
Cckar??[Similar Products]
NCBI Official Synonym Symbols
AW106902
??[Similar Products]
NCBI Protein Information
cholecystokinin receptor type A
UniProt Protein Name
Cholecystokinin receptor type A
UniProt Synonym Protein Names
Cholecystokinin-1 receptor; CCK1-R
Protein Family
Cholecystokinin receptor
UniProt Gene Name
Cckar??[Similar Products]
UniProt Synonym Gene Names
CCK-A receptor; CCK-AR; CCK1-R??[Similar Products]
UniProt Entry Name
CCKAR_MOUSE
UniProt Comments for Cckar
CCKAR: Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; endosome; integral to membrane; lysosome; membrane; plasma membrane; terminal button
Molecular Function: cholecystokinin receptor activity; G-protein coupled receptor activity; signal transducer activity
Biological Process: actin cytoskeleton reorganization; axonogenesis; digestion; eating behavior; elevation of cytosolic calcium ion concentration; feeding behavior; forebrain development; G-protein coupled receptor protein signaling pathway; gastric acid secretion; insulin secretion; neuron migration; pancreatic juice secretion; reduction of food intake in response to dietary excess; regulation of calcium ion transport; regulation of hormone secretion; regulation of potassium ion transport; response to heat; response to starvation; signal transduction; thermoregulation
Research Articles on Cckar
1. There is functional synergy between cholecystokinin receptors CCKAR and CCKBR in mammalian brain development.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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