CAF-1; CAF1; CAF1B; CAF1P150; P150; Chromatin assembly factor I p150 subunit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Bovine CHAF1A. No significant cross-reactivity or interference between Bovine CHAF1A and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CHAF1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS284901 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Chromatin assembly factor 1 subunit A (CHAF1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHAF1A. The ELISA analytical biochemical technique of the MBS284901 kit is based on CHAF1A antibody-CHAF1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHAF1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHAF1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CHAF1A in samples. An antibody specific for CHAF1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHAF1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHAF1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHAF1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

107,258 Da

chromatin assembly factor 1 subunit A

chromatin assembly factor 1 subunit A

Chromatin assembly factor 1 subunit A

CAF; CAF1P150; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150??[Similar Products]

Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.

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